PMID: 2112260Jan 1, 1990Paper

Stacking of three different restriction and modification systems in Lactococcus lactis by cotransformation

Plasmid
J Josephsen, T R Klaenhammer

Abstract

Four plasmids encoding restriction and modification (R/M) systems are described that are different in the specificity of their restrictive activity toward the small isometric phage p2 and prolate phage c2. The R/M plasmids were cotransformed into Lactococcus lactis MG1363 with pVS2, encoding resistance to chloramphenicol and erythromycin, to indicate successful transformation events. Analysis of cotransformants showed that three different R/M plasmids could be combined in L. lactis MG1363. The efficiency at which phage plaqued on the transformants decreased as the number of R/M plasmids increased. Some plasmid combinations were unstable suggesting replicon incompatibility.

References

Mar 1, 1988·Biochimie·M E Sanders

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Citations

Dec 1, 1995·Molecular Biotechnology·P K Dinsmore, T R Klaenhammer
May 19, 1995·Gene·N NyengaardJ Josephsen
Jul 10, 2001·Letters in Applied Microbiology·N CorneauE Emond
Nov 30, 2007·Journal of Applied Microbiology·Jitka Holubová, Jytte Josephsen
Apr 2, 1999·Applied and Environmental Microbiology·A FordeG F Fitzgerald
Jul 31, 1998·Journal of Bacteriology·B KochK Hammer
Feb 1, 1993·Applied and Environmental Microbiology·W D Sing, T R Klaenhammer
Jul 1, 1993·Applied and Environmental Microbiology·B MayoG Venema

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