Stage-specific expression of three cell surface carbohydrate antigens during murine spermatogenesis detected with monoclonal antibodies

Developmental Biology
B A FendersonE M Eddy


We have identified three germ cell surface carbohydrate antigens that exhibit a common, stage-specific pattern of expression during spermatogenesis in the mouse. IgM-class monoclonal antibodies designated "J1," "C6," and "A5" were absorbed by adult testis, but not by any adult somatic tissue tested. In indirect immunofluorescence assays using collagenase-dissociated prepuberal and adult testicular cells, these antibodies labeled the surfaces of early and late pachytene spermatocytes and round spermatids. Gonocytes from fetal and neonatal testes were not labeled. In paraffin sections of prepuberal and adult testes, sialidase treatment exposed antigens recognized by antibodies C6 and A5 on preleptotene, leptotene, and zygotene spermatocytes located near the perimeter of seminiferous tubules. The determinants recognized by antibodies J1, C6, and A5 were characterized partially using a sugar hapten inhibition assay. The binding of J1 to adult testicular cells was inhibited specifically by N-acetylglucosamine and the binding of both C6 and A5 was inhibited by N-acetyllactosamine. The glycoconjugates recognized by J1, C6, and A5 eluted from gel filtration columns with an apparent molecular weight greater than 1 X 10(6) and were sensi...Continue Reading


Dec 24, 1997·Molecular Reproduction and Development·A T DowsingA O Trounson
Mar 29, 2000·Molecular Reproduction and Development·N N AtanassovaC A Valkova
Nov 1, 1992·Molecular Reproduction and Development·D WatanabeY Nishimune
Feb 1, 1995·Molecular Reproduction and Development·U KoshimizuY Nishimune
Jul 19, 2000·Archives of Biochemistry and Biophysics·A Abou-Haila, D R Tulsiani
Jun 1, 1985·Developmental Biology·M De FeliciG Cossu
Mar 1, 1986·Developmental Biology·B A FendersonS Hakomori
Dec 1, 1984·Cell Differentiation·M VojtískováZ Pokorná
Nov 1, 1987·Journal of Reproductive Immunology·A Jassim, H Festenstein
May 1, 1989·Journal of Reproductive Immunology·O MikamiJ Furuyama
Oct 3, 2002·Biochemical and Biophysical Research Communications·Jürgen NeesenIrene Mendoza-Lujambio
Jul 4, 2009·Reproductive Biomedicine Online·Jinlian HuaKuldip S Sidhu
Aug 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·P Dráber, P Malý
Sep 29, 2009·Journal of Molecular Cell Biology·Zhuo YuLixin Feng
Dec 17, 2009·Journal of Molecular Cell Biology·Candace L Kerr, Linzhao Cheng
Feb 26, 2010·Biology of Reproduction·Michaela GruberM Celeste Simon
Apr 17, 2008·Reproductive Biomedicine Online·Zsolt Peter NagyChing-Chien Chang
May 14, 2005·Molecular Reproduction and Development·Matthew J KohnMelvin L DePamphilis
Jan 1, 1987·Annals of the New York Academy of Sciences·C F MilletteG L Gerton
Jul 1, 1988·Differentiation; Research in Biological Diversity·B A FendersonS Hakomori
Jan 24, 2007·Reproductive Biology and Endocrinology : RB&E·Wilhelm BoneAarnoud C van der Spoel
Mar 3, 2007·Developmental Biology·Richard I SherwoodDouglas A Melton
Jun 14, 2005·Developmental Biology·Masanobu ShojiNorio Nakatsuji
Jan 12, 2007·Biology of Reproduction·Makoto C Nagano
Oct 6, 2000·Genesis : the Journal of Genetics and Development·K C PfendlerP M Iannaccone


Jan 1, 1977·Immunological Reviews·F Jacob
Jul 1, 1977·The Journal of Cell Biology·C F Millette, A R Bellvé
Jan 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·K B BechtolR H Kennett
May 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·T MuramatsuF Jacob
Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Feb 1, 1977·Developmental Biology·M G O'Rand, L J Romrell
Jul 1, 1977·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·A R BellvéD A O'Brien
Oct 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·K ArtztF Jacob
Feb 1, 1980·Cell·C C Cheng, D Bennett
Jan 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·M VojtískováP Dráber
Jun 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·M FukudaS Hakomori
Nov 1, 1982·The Journal of Cell Biology·B D Shur, N G Hall
Feb 4, 1983·Science·G M Edelman
May 1, 1981·Archives of Andrology·J K Koehler
Jan 1, 1981·Annual Review of Biochemistry·S Hakomori
Jan 1, 1981·Journal of Supramolecular Structure and Cellular Biochemistry·C G Glabe, W J Lennarz
Aug 1, 1962·Biological Reviews of the Cambridge Philosophical Society·E C ROOSEN-RUNGE

Related Concepts

Monoclonal Antibodies
Monoclonal antibodies, antineoplastic
Immunofluorescence Assay
Benign Neoplasm of Testis
Keratan-sulfate endo-1,4-b-galactosidase
Neoplasm of Uncertain or Unknown Behavior of Testis
Sexual Maturation

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Sexual Dimorphism in Neurodegeneration

There exist sex differences in neurodevelopmental and neurodegenerative disorders. For instance, multiple sclerosis is more common in women, whereas Parkinson’s disease is more common in men. Here is the latest research on sexual dimorphism in neurodegeneration

HLA Genetic Variation

HLA genetic variation has been found to confer risk for a wide variety of diseases. Identifying these associations and understanding their molecular mechanisms is ongoing and holds promise for the development of therapeutics. Find the latest research on HLA genetic variation here.

Super-resolution Microscopy

Super-resolution microscopy is the term commonly given to fluorescence microscopy techniques with resolutions that are not limited by the diffraction of light. Here are the latest discoveries pertaining to super-resolution microscopy.

Genetic Screens in iPSC-derived Brain Cells

Genetic screening is a critical tool that can be employed to define and understand gene function and interaction. This feed focuses on genetic screens conducted using induced pluripotent stem cell (iPSC)-derived brain cells.

Brain Lower Grade Glioma

Low grade gliomas in the brain form from oligodendrocytes and astrocytes and are the slowest-growing glioma in adults. Discover the latest research on these brain tumors here.

CD4/CD8 Signaling

Cluster of differentiation 4 and 8 (CD8 and CD8) are glycoproteins founds on the surface of immune cells. Here is the latest research on their role in cell signaling pathways.

Alignment-free Sequence Analysis Tools

Alignment-free sequence analyses have been applied to problems ranging from whole-genome phylogeny to the classification of protein families, identification of horizontally transferred genes, and detection of recombined sequences. Here is the latest research.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.