Mar 27, 2020

Stagewise keratinocyte differentiation from human embryonic stem cells by defined signal transduction modulators

International Journal of Biological Sciences
Hui ZhongGuokai Chen

Abstract

Keratinocyte is the predominant cell type in the epidermis of skin, and it provides the protective barrier function for the body. Various signaling pathways have been implicated in keratinocyte differentiation in animal models; However, their temporal regulation and interactions are still to be explored in pluripotent stem cell models. In this report, we use human embryonic stem cells to demonstrate that epidermal ectoderm and subsequent keratinocyte cell fate can be determined step by step under the regulation of defined factors. The inhibition of TGFβ initiates ectodermal lineage differentiation, and the activation of BMP pathway drives epidermal TP63 expression. Meanwhile, the timely activation of WNT pathway suppresses extraembryonic lineage, and promotes epidermal cell fate. With further specification by NOTCH inhibition, more than 90% of cells become TP63-positive stage Ⅱ keratinocytes. Finally, stage Ⅲ keratinocytes are produced under defined hypo-calcium keratinocyte culture conditions, and are further matured in mouse xenograft model. This study not only establishes an in vitro platform to study keratinocyte cell fate determination, but also provides an efficient protocol to produce keratinocytes for disease models and...Continue Reading

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Mentioned in this Paper

Protein Expression
Mouse Model
Regulation of Biological Process
Keratinocyte
Skin
Keratinocyte Differentiation
Bone morphogenetic protein 2
Determination Aspects
WNT1
Inhibition of Notch Signaling Pathway

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