PMID: 6162250Sep 1, 1980Paper

Staining and histochemistry of undecalcified bone embedded in a water-miscible plastic

Stain Technology
R M Franklin, M T Martin

Abstract

Undecalcified bone fixed in a variety of fixatives and embedded in a new formulation of 2-hydroxypropyl methacrylate at 4 c has been sectioned at 1 to 5 microns. The embedding mixture contains 2-butoxyethanol as plasticizer and triethyleneglycol dimethacrylate as cross-linker. The accelerator was benzoyl peroxide and the catalyst was N,N-dimethylaniline. With proper embedding and care in sectioning it is possible to obtain sections with relatively little bone compression, excellent preservation of cellular elements, and a minimum of wrinkling. A wide variety of stains have been used for these sections and those reported here are Gill's hematoxylin-eosin, Nocht's azure-eosin, Feulgen, Hoechst 33258 (bisbenzimid H 33258), methyl green-pyronin, PAS, alizarin red, and von Kossa silver stain. There was excellent preservation of acid and alkaline phosphatase activities. A new method of prestaining immunofluorescent labeling was also applied to bone and examples of staining with anticollagen I and antifibronectin are presented.

References

Nov 15, 1979·Analytical Biochemistry·C F CesaroneL Santi
Mar 1, 1979·Journal of Microscopy·W J Swartz, F R Nusbickel
Jan 1, 1979·International Review of Cytology·R M Frank
May 1, 1979·The Histochemical Journal·J J Johnstone
Jul 1, 1965·The Journal of Cell Biology·E H Leduc, S J Holt

Citations

Jan 1, 1986·Archives of Virology·J BöniR M Franklin
Nov 24, 2004·Der Orthopäde·M JagodzinskiH Mertisching
Mar 1, 1982·Stain Technology·G H KennerJ B Park
Nov 1, 1983·Stain Technology·R M Franklin, M T Martin
Jan 1, 1987·Stain Technology·M Hott, P J Marie
Sep 15, 1998·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·B F Romeike, W Feiden
Jan 1, 1985·Progress in Histochemistry and Cytochemistry·J A Litwin
Jan 1, 1983·Annals of the New York Academy of Sciences·R M Franklin
Oct 4, 2005·Acta Biologica Hungarica·L G PuskásJ Gy Papp
Jan 1, 1983·Hybridoma·J R Pink, A M Rijnbeek
Nov 13, 2004·Endocrinology·Elisabetta GazzerroErnesto Canalis
Dec 17, 1985·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·N M Le Douarin
Oct 1, 1982·European Journal of Immunology·J S NowakR M Franklin
Jul 1, 1982·Acta Pathologica, Microbiologica, Et Immunologica Scandinavica. Section A, Pathology·C D BirchM E Jensen

Related Concepts

Skeletal Bone
Chick Embryo
Zyderm
Fibronectins
Cytochemistry
Histological Techniques
Ultramicrotomy
Phosphoric Monoester Hydrolases
Staining and Labeling

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