Staining efficacy assessment of a differential routine and special stains for pathological stromal calcifications in maxillofacial lesions.

Journal of Histotechnology
ShefaliSaurabh Juneja

Abstract

Head and neck connective tissue lesions may have diverse calcifications within the fibrous connective tissue stroma. The perplexity involved in the identification and determination of the nature or degree of calcification through routine hematoxylin and eosin (H&E) stains necessitates the usage of a specific, simple, and cost- and time-effective differential staining techniques. The aim of the present study was to develop criteria to distinguish bone formation from bone resorption using methylene blue-acid fuchsin (MB/AF) stain and the role of collagen fibers in the identification of stromal calcifications using polarizing microscopy with picrosirius red stain. Twenty cases with pathological diagnoses for various stromal calcifications in maxillofacial lesions were retrieved from the departmental archives. Decalcified formalin fixed paraffin embedded tissue sections were stained with hematoxylin and eosin, Masson's trichrome (MT), methylene blue-acid fuchsin (MB/AF), and picrosirius red. The stained sections were assessed to identify the calcifications found in the surrounding connective tissue stroma. It was observed that most cases showed maximum staining intensity with MB/AF stain as compared to the other staining methods. M...Continue Reading

References

Nov 11, 2011·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·K GuptaV S Kotrashetti
Feb 17, 2017·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Johanna WarshawDonald H Enlow

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