Staining of endothelial cells does not change the result of cell density

Cell and Tissue Banking
Daniel A WenzelMaximilian Schultheiss

Abstract

To determine the corneal endothelial cell density human donor corneas are usually placed in hypotonic balanced salt solution for unstained cell counting. Experimental studies often stain the corneal endothelium to highlight cells and cell borders and simplify counting. However, staining normally terminates the experiments. Up to date, there is no publication comparing endothelial cell counts before and after staining. This study compared the counting results of unstained (hypotonic balanced salt solution) and stained (trypan blue & alizarin red S) assessment of the corneal endothelial cell density. No evidence for significantly differing cell counts was found between unstained and stained analysis of the corneal endothelial cell density. Therefore, we consider unstained counting non-inferior to stained counting.

References

Apr 26, 2006·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Christian MeltendorfJan Schroeter
Jun 6, 2009·Developments in Ophthalmology·Jan Schroeter, Peter Rieck
Oct 29, 2017·Cell and Tissue Banking·Berenike C KunzmannMaximilian Schultheiss

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Citations

Aug 24, 2019·Nature Structural & Molecular Biology·Robert G Roeder
Aug 30, 2019·Nature·Patrick Cramer

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Methods Mentioned

BETA
light microscopy

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