Staining proteins in gels

Current Protocols in Immunology
Joachim Sasse, Sean R Gallagher

Abstract

This unit describes protocols for detecting protein in a gel by either Coomassie blue, silver or fluorescent staining. Alternate rapid staining procedures are provided for each method and a support protocol describes how to photograph stained gels. Fluorescent staining (e.g., with SYPRO Orange or Red) is described as a popular alternative to traditional staining procedures, mainly because it is more sensitive than Coomassie staining, and often as sensitive as silver staining. Staining of proteins in SDS-polyacrylamide gels is described, and an alternate protocol details variations in the procedure for proteins in nondenaturing gels. A final support protocol describes the photography of fluorescently stained proteins.

References

Jul 1, 1980·Analytical Biochemistry·B R OakleyN R Morris
Jan 1, 1983·Methods in Enzymology·C M Wilson
Jan 1, 1984·Methods in Enzymology·C R MerrilM L Van Keuren
Aug 1, 1996·Analytical Biochemistry·T H SteinbergV L Singer
May 15, 1997·Analytical Biochemistry·T H SteinbergV L Singer

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Citations

Sep 18, 2013·Molecular & Cellular Proteomics : MCP·R Hussain Butt, Jens R Coorssen
Mar 26, 2010·Journal of Virology·Perdita BackesVolker Lohmann
Nov 26, 2008·Liver Transplantation : Official Publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society·Mehmet UzunelSuchitra Sumitran-Holgersson
Jun 29, 2010·Journal of Bacteriology·Abdelali DaddaouaJuan-Luis Ramos
Sep 5, 2019·Current Protocols in Immunology·Jolien J LuimstraHuib Ovaa

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