Standard sub-thermoneutral caging temperature influences radiosensitivity of hematopoietic stem and progenitor cells

PloS One
Benjamin J PovinelliMichael J Nemeth

Abstract

The production of new blood cells relies on a hierarchical network of hematopoietic stem and progenitor cells (HSPCs). To maintain lifelong hematopoiesis, HSPCs must be protected from ionizing radiation or other cytotoxic agents. For many years, murine models have been a valuable source of information regarding factors that either enhance or reduce the survival of HSPCs after exposure of marrow to ionizing radiation. In a recent series of studies, however, it has become clear that housing-related factors such as the cool room temperature required for laboratory mice can exert a surprising influence on the outcome of experiments. Here we report that the mild, but chronic cold-stress endured by mice housed under these conditions exerts a protective effect on HSPCs after both non-lethal and lethal doses of total body irradiation (TBI). Alleviation of this cold-stress by housing mice at a thermoneutral temperature (30°C) resulted in significantly greater baseline radiosensitivity to a lethal dose of TBI with more HSPCs from mice housed at thermoneutral temperature undergoing apoptosis following non-lethal TBI. Cold-stressed mice have elevated levels of norepinephrine, a key molecule of the sympathetic nervous system that binds to β...Continue Reading

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Citations

Apr 30, 2019·Journal of Cellular Physiology·Yanan KongYu Zhao
Jan 24, 2019·The Journal of Immunology : Official Journal of the American Association of Immunologists·Bonnie L HylanderElizabeth A Repasky
Oct 24, 2020·ILAR Journal·Vanessa K LeeMichael J Huerkamp
Dec 18, 2020·Frontiers in Immunology·Fiorella Vialard, Martin Olivier

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Methods Mentioned

BETA
enzyme-linked immunosorbent assays
flow cytometry
flow
ELISA
irradiated

Software Mentioned

GraphPad Prism
GraphPad
GraphPadv6

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