Standardization and Optimization of Intraoperative Molecular Imaging for Identifying Primary Pulmonary Adenocarcinomas

Molecular Imaging and Biology : MIB : the Official Publication of the Academy of Molecular Imaging
Jarrod PredinaSunil Singhal

Abstract

Intraoperative molecular imaging (IMI) is an emerging technology used to locate pulmonary adenocarcinomas and identify positive margins during surgery. Background noise and tissue autofluorescence have been major obstacles. The goal of this study is to optimize the image quality of folate receptor alpha (FRα) targeted IMI for pulmonary adenocarcinomas by modifying emission data. A total of 15 lung cancer patients were enrolled in a pilot study. In the first cohort, FRα upregulation within pulmonary adenocarcinoma tumors was confirmed by analyzing specimens from five pulmonary adenocarcinoma patients with flow cytometry and immunohistochemistry. Next, in a cohort of five additional patients, autofluorescence of intrathoracic structures and tissues was quantified. Lastly, five patients with tumors at various depths from the pleural surface were enrolled and received the FRα-targeted optical contrast agent, EC17. In this final cohort, resected pulmonary adenocarcinomas were imaged at a wide range of fluorescence exposure times (0 to 200 ms), various laser powers, and with unique filter configurations. Tumor-to-noise ratio (TNR) for images was generated using region of interest software. Pulmonary adenocarcinomas highly express FRα...Continue Reading

References

Jan 1, 1994·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·L T MantovaniM I Colnaghi
May 16, 2002·Bioconjugate Chemistry·Yuhui LinChing-Hsuan Tung
Apr 20, 2004·Advanced Drug Delivery Reviews·Philip S Low, Asok C Antony
Apr 20, 2004·Advanced Drug Delivery Reviews·Yingjuan LuPhilip S Low
Oct 12, 2005·Biotechnology Annual Review·Monica Monici
Oct 7, 2006·American Journal of Respiratory and Critical Care Medicine·Luc ThibervilleGenevieve Bourg Heckly
Jan 30, 2007·The Annals of Thoracic Surgery·Hiroshi SugimuraPeter C Pairolero
Feb 14, 2009·The European Respiratory Journal·L ThibervilleG Bourg-Heckly
May 8, 2009·Current Opinion in Chemical Biology·Philip Stewart Low, Sumith Anurasiri Kularatne
Jul 30, 2010·Journal of Medicinal Chemistry·Wei Xia, Philip S Low
Dec 17, 2010·Annals of Surgical Oncology·Louis A AlipertiSunil Singhal
Aug 31, 2012·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Brian MadajewskiSunil Singhal
May 25, 2013·Surgical Oncology·David FedorSunil Singhal
Jun 25, 2013·Cancer·Joost R van der VorstAlexander L Vahrmeijer
Dec 21, 2013·Technology in Cancer Research & Treatment·O T OkusanyaS Singhal
Oct 23, 2015·International Journal of Molecular Imaging·Elizabeth De JesusSunil Singhal
Nov 7, 2015·The Journal of Urology·Thomas J GuzzoSunil Singhal
Nov 14, 2015·Scientific Reports·Ryan P JudySunil Singhal

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Citations

Jan 10, 2018·Frontiers in Oncology·Tadanobu NagayaHisataka Kobayashi
Dec 7, 2019·Journal of Biomedical Optics·John Y K LeeSunil Singhal
Feb 15, 2020·Scientific Reports·Jian XuShiyang Pan
Aug 12, 2018·Journal of Surgical Oncology·Andrew D NewtonSunil Singhal
Nov 23, 2020·The Annals of Thoracic Surgery·Sidhu GangadharanSunil Singhal

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