Standardization and quality assessment for clinical grade mesenchymal stem cells from human adipose tissue

Hematology, Transfusion and Cell Therapy
Tanya Debnath, Lakshmi Kiran Chelluri

Abstract

Mesenchymal stem cells have immense potential in stem cell-based therapies, however there is a pre-requisite to develop a curative cell dose. Adipose tissue-derived mesenchymal stem cells are promising mainly due to their potential abundance, immunomodulatory effect and remarkable differentiation potential. Nevertheless, senescence may develop during their in vitro expansion due to the incidence of genetic instability. Hence, it is important to attain an ideal balance between mesenchymal stem cell growth, quality and genetic integrity before their clinical use. Stromal vascular fraction was obtained from omentum tissue of patients undergoing liposuction procedures for morbid obesity. This study standardized a closed system protocol which can be utilized for clinical grade stem cell derivation. Stages of cell growth and characterization of human adipose tissue-derived mesenchymal stem cells were also assessed along with the chromosomal stability in these in vitro cultures. Human adipose tissue-derived mesenchymal stem cells maintained their spindle-shaped morphology and were able to proliferate and renew, confirming their suitability for in vitro cultivation and generate clinical grade mesenchymal stem cells. Immunophenotyping i...Continue Reading

Citations

Feb 23, 2020·European Journal of Trauma and Emergency Surgery : Official Publication of the European Trauma Society·Liudmila LeppikJohn Howard Barker
Dec 6, 2019·Stem Cell Research & Therapy·Nuria Nieto-NicolauRicardo P Casaroli-Marano
Mar 13, 2020·Cellular & Molecular Biology Letters·Jing ZhangXingbin Hu
Oct 11, 2020·BioFactors·Simin SharifiMohammad Samiei
Nov 17, 2020·Frontiers in Veterinary Science·Patrícia de Faria LainettiCarlos Eduardo Fonseca-Alves
Nov 14, 2020·International Journal of Molecular Sciences·Antonio Martinez-LopezSalvador Arias-Santiago
May 8, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Ruijie WangWenhui Wu
Jun 3, 2021·Polymers·Sybele SaskaJamil Awad Shibli

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Methods Mentioned

BETA
flow cytometry

Software Mentioned

Leica
Cell Quest

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