Standardization of a cytometric p24-capture bead-assay for the detection of main HIV-1 subtypes

Journal of Virological Methods
Mélanie MerbahAgnès-Laurence Chenine

Abstract

The prevailing method to assess HIV-1 replication and infectivity is to measure the production of p24 Gag protein by enzyme-linked immunosorbent assay (ELISA). Since fluorescent bead-based technologies offer a broader dynamic range and higher sensitivity, this study describes a p24 capture Luminex assay capable of detecting HIV-1 subtypes A-D, circulating recombinant forms (CRF) CRF01_AE and CRF02_AG, which together are responsible for over 90% of HIV-1 infections worldwide. The success of the assay lies in the identification and selection of a cross-reactive capture antibody (clone 183-H12-5C). Fifty-six isolates that belonged to six HIV-1 subtypes and CRFs were successfully detected with p-values below 0.021; limits of detection ranging from 3.7 to 3 × 104 pg/ml. The intra- and inter-assay variation gave coefficient of variations below 6 and 14%, respectively. The 183-bead Luminex assay also displayed higher sensitivity of 91% and 98% compared to commercial p24 ELISA and a previously described Luminex assay. The p24 concentrations measured by the 183-bead Luminex assay showed a significant correlation (R=0.92, p<0.0001) with the data obtained from quantitative real time PCR. This newly developed p24 assay leverages the advant...Continue Reading

References

Dec 31, 2002·Journal of Virological Methods·Stefan A KleinWilli K Roth
Dec 4, 2003·International Archives of Allergy and Immunology·Jörg Schüpbach
Jun 15, 2004·Molecular Therapy : the Journal of the American Society of Gene Therapy·Laurent M HumeauBoro Dropulic
Dec 23, 2008·Journal of Virological Methods·Angélique BiancottoJean-Charles Grivel
Feb 8, 2011·AIDS·Joris HemelaarUNKNOWN WHO-UNAIDS Network for HIV Isolation and Characterisation
Nov 13, 2012·The Journal of Clinical Investigation·R Brad JonesMario A Ostrowski
Feb 15, 2013·PLoS Pathogens·Andrea IntroiniLeonid Margolis
Apr 2, 2013·Proceedings of the National Academy of Sciences of the United States of America·Nicholas F ParrishBeatrice H Hahn
Aug 13, 2013·PLoS Pathogens·Jan BalzariniDominique Schols
Jan 15, 2015·Retrovirology·Lise ChauveauOlivier Schwartz

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Citations

Aug 2, 2017·Advanced Pharmaceutical Bulletin·Mohammad Tasyriq Che Omar

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