Standardization of CTC AR-V7 PCR assay and evaluation of its role in castration resistant prostate cancer progression

The Prostate
Stefania TommasiEmanuele Naglieri

Abstract

Castration resistant prostate cancer (CRPC) represents the most aggressive status of this neoplastic disease, also characterized by the absence of biomarkers predictive of clinical outcome. New drugs as abiraterone or enzalutamide, affecting androgen receptor pathway at different levels, inhibit the proliferative advantage of prostate cancer cells with important long term benefits. Despite the advantages of this second-generation androgen deprivation therapy (ADT), resistance mechanisms, primitive or acquired, often develop. The existence of androgen receptor (AR) splice variants (AR-Vs), in particular AR-V7 expression detected in circulating tumor cells (CTCs), represents an example of acquired resistance, as evidenced in preclinical and clinical studies. Recent studies also have suggested the role of AR-V7 as a prognostic biomarker in mCRPC. In this field, hot topics are the methodology used to isolate CTC and the assay for AR-V7 measurement. Our study aims to develop a standardized operating procedure (SOP) to evaluate AR-V7 in CRPC. The application of a realized cell based Reference Sample as Standardized Quality Control tool for CTC-AR-V7 assay has been shown. Then the development, the performance evaluation and contextual...Continue Reading

References

May 27, 2011·The New England Journal of Medicine·Johann S de BonoUNKNOWN COU-AA-301 Investigators
Aug 17, 2012·The New England Journal of Medicine·Howard I ScherUNKNOWN AFFIRM Investigators
Dec 11, 2013·European Urology·Axel HeidenreichUNKNOWN European Association of Urology
Jul 23, 2014·Hormones & Cancer·Mary NakazawaJun Luo
Sep 4, 2014·The New England Journal of Medicine·Emmanuel S AntonarakisJun Luo
May 18, 2016·Prostate Cancer and Prostatic Diseases·E S AntonarakisJ Luo

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Citations

Jan 4, 2019·Clinical Chemistry·Klaus PantelHoward I Scher
Jan 31, 2019·Diagnostics·Jochen Neuhaus, Bo Yang
Nov 13, 2019·Molecular Aspects of Medicine·Edward Geeurickx, An Hendrix

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