Standardization of ELISA protocols for serosurveys of the SARS-CoV-2 pandemic using clinical and at-home blood sampling

Nature Communications
Carleen Klumpp-ThomasKaitlyn Sadtler

Abstract

The extent of SARS-CoV-2 infection throughout the United States population is currently unknown. High quality serology is key to avoiding medically costly diagnostic errors, as well as to assuring properly informed public health decisions. Here, we present an optimized ELISA-based serology protocol, from antigen production to data analyses, that helps define thresholds for IgG and IgM seropositivity with high specificities. Validation of this protocol is performed using traditionally collected serum as well as dried blood on mail-in blood sampling kits. Archival (pre-2019) samples are used as negative controls, and convalescent, PCR-diagnosed COVID-19 patient samples serve as positive controls. Using this protocol, minimal cross-reactivity is observed for the spike proteins of MERS, SARS1, OC43 and HKU1 viruses, and no cross reactivity is observed with anti-influenza A H1N1 HAI. Our protocol may thus help provide standardized, population-based data on the extent of SARS-CoV-2 seropositivity, immunity and infection.

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Citations

Mar 17, 2021·Journal of Clinical Immunology·Jennifer HicksKaitlyn Sadtler
Mar 23, 2021·Clinical & Translational Immunology·Alana L WhitcombeNicole J Moreland
Apr 4, 2021·International Journal of Molecular Sciences·Linhua TianLili Wang
Mar 16, 2021·JCI Insight·Abdelilah MajdoubiPascal M Lavoie
May 6, 2021·Scientific Reports·Melissa M MatthewsMatthias Wolf
Jun 24, 2021·Science Translational Medicine·Heather KalishKaitlyn Sadtler
Jul 2, 2021·Journal of Virological Methods·Suraj JagtapRahul Roy

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Methods Mentioned

BETA
enzyme-linked immunosorbent assays
ELISA
size-exclusion chromatography
transmission electron microscopy
PCR
ELISAs
transfection
flow filtration
electrophoresis

Clinical Trials Mentioned

NCT04334954
NCT01386424

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