Standardization of Flow Cytometric Immunophenotyping for Hematological Malignancies: The FranceFlow Group Experience

Cytometry. Part a : the Journal of the International Society for Analytical Cytology
Françoise SollyLudovic Lhermitte

Abstract

Flow cytometry is broadly used for the identification, characterization, and monitoring of hematological malignancies. However, the use of clinical flow cytometry is restricted by its lack of reproducibility across multiple centers. Since 2006, the EuroFlow consortium has been developing a standardized procedure detailing the whole process from instrument settings to data analysis. The FranceFlow group was created in 2010 with the intention to educate participating centers in France about the standardized instrument setting protocol (SOP) developed by the EuroFlow consortium and to organise several rounds of quality controls (QCs) in order to evaluate the feasibility of its application and its results. Here, we report the 5 year experience of the FranceFlow group and the results of the seven QCs of 23 instruments, involving up to 19 centers, in France and in Belgium. The FranceFlow group demonstrates that both the distribution and applicability of the SOP have been successful. Intercenter reproducibility was evaluated using both normal and pathological blood samples. Coefficients of variation (CVs) across the centers were <7% for the percentages of cell subsets and <30% for the median fluorescence intensities (MFIs) of the mark...Continue Reading

References

Oct 10, 1997·American Journal of Clinical Pathology·E J MoreauD Catovsky
Jan 17, 2008·Blood·Fiona E Craig, Kenneth A Foon
May 4, 2012·Leukemia·J J M van DongenUNKNOWN EuroFlow Consortium (EU-FP6, LSHB-CT-2006-018708)
Sep 6, 2012·Leukemia·T KalinaUNKNOWN EuroFlow Consortium (EU-FP6, LSHB-CT-2006-018708)
Oct 24, 2013·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Françoise SollyMarie C Béné
Mar 14, 2014·British Journal of Haematology·Ulrika JohanssonUNKNOWN British Committee for Standards in Haematology
Oct 28, 2014·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Tomas KalinaAlberto Orfao
Jun 24, 2015·Cytometry. Part B, Clinical Cytometry·Frank W M B PreijersUNKNOWN Section Immunological and Molecular Cell Diagnostics of the Foundation for Quality Assessment in Medical Laboratories(SKML)
Nov 21, 2017·Journal of Immunological Methods·Michaela NovákováTomáš Kalina

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Citations

Nov 27, 2019·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Fabienne LucasPeter Lopez
Apr 3, 2020·British Journal of Haematology·Diana HanekampJacqueline Cloos
Oct 2, 2020·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Ludovic LhermitteUNKNOWN EuroFlow Consortium
Aug 18, 2020·Cytometry. Part B, Clinical Cytometry·Agathe DebliquisBernard Drenou
Feb 10, 2021·Blood Advances·Nabih MaslahGuillaume P Andrieu
Oct 2, 2020·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·M VassalloJ Durant
Oct 16, 2021·Frontiers in Cell and Developmental Biology·Carmen-Mariana AaneiLydia Campos Catafal

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