PMID: 3770051Aug 1, 1986Paper

Standardization of steroid receptor assays in human breast cancer--III. Selection of reference material for intra- and inter-laboratory quality control

European Journal of Cancer & Clinical Oncology
S M Thorpe, A Koenders

Abstract

A comparison of estrogen and progesterone receptor (ER and PgR, respectively) analyses using minced frozen tissue and lyophilized cytosols of the same samples demonstrated that intra-laboratory variations in assays performed by 13 members of the EORTC Receptor Group are lowest using the lyophilized samples. Inter-laboratory variation in receptor values was on the same order of magnitude for both types of samples (ca 22% for ER and 30% for PgR). There was no correlation between receptor values measured within each laboratory for either ER or PgR in minced tissues compared to lyophilized cytosols, which illustrates that methods of tissue disruption, extraction of receptors, and preparation of cytosol are sources of intra- and inter-laboratory variation. In some laboratories the handling of the tissue was apparently sub-optimal since a slight but significant difference was found in the overall mean concentration of ER in minced tissue compared to lyophilized cytosol samples. It was concluded that lyophilized tissue samples are the material of choice for routine intra- and inter-laboratory quality controls. However, differences in methods of handling tissue to obtain cytosol should not be disregarded since they lead to increased in...Continue Reading

References

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Citations

Oct 1, 1989·Breast Cancer Research and Treatment·M GrimauxH Magdelénat
Jan 1, 1995·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·S RomainH Magdelenat
Jan 1, 1994·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·S RomainP M Martin
Oct 1, 1994·International Journal of Cancer. Journal International Du Cancer·S RomainP M Martin
Feb 15, 1990·International Journal of Cancer. Journal International Du Cancer·M GrimauxH Magdelenat
May 1, 1987·European Journal of Cancer & Clinical Oncology·G Leclercq
Jan 1, 1992·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·M Roux-DossetoP M Martin
Oct 3, 2018·Obesity·Rebecca KuriyanAnura V Kurpad
Apr 6, 2013·The International Journal of Biological Markers·Rosella SilvestriniMaria Grazia Daidone
Jan 1, 1991·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·S RomainP M Martin

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