Nov 26, 2016

Standardized experiments in mutant mice reveal behavioural similarity on 129S5 and C57BL/6J backgrounds

Genes, Brain, and Behavior
Louie N van de LagemaatSeth G N Grant

Abstract

Behavioural analysis of mice carrying engineered mutations is widely used to identify roles of specific genes in components of the mammalian behavioural repertoire. The reproducibility and robustness of phenotypic measures has become a concern that undermines the use of mouse genetic models for translational studies. Contributing factors include low individual study power, non-standardized behavioural testing, failure to address confounds and differences in genetic background of mutant mice. We have examined the importance of these factors using a statistically robust approach applied to behavioural data obtained from three mouse mutations on 129S5 and C57BL/6J backgrounds generated in a standardized battery of five behavioural assays. The largest confounding effect was sampling variation, which partially masked the genetic background effect. Our observations suggest that strong interaction of mutation with genetic background in mice in innate and learned behaviours is not necessarily to be expected. We found composite measures of innate and learned behaviour were similarly impacted by mutations across backgrounds. We determined that, for frequently used group sizes, a single retest of a significant result conforming to the com...Continue Reading

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Citations

Mentioned in this Paper

Study
Behavior, Animal
Size
Genes
Impacted Tooth
Harassment, Non-Sexual
Evaluation
Behavioral Test
Computer Programs and Programming
Abnormal Behavior

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