Standardized GMP-compliant scalable production of human pancreas organoids.

Stem Cell Research & Therapy
Marta DossenaLorenza Lazzari

Abstract

Organoids are three-dimensional in vitro-grown cell clusters that recapitulate key features of native organs. In regenerative medicine, organoid technology represents a promising approach for the replacement of severely damaged organs, such as the pancreas in patients with type 1 diabetes. Isolation human pancreas organoids (hPOs) in chemically defined serum-free culture media would be a major milestone for this approach. Starting from discarded pancreatic tissues, we developed a large-scale process for obtaining clinically relevant quantities of undifferentiated organoids, obviating enzymatic digestion and operator-dependent pancreatic ducts picking steps. hPO identity was characterized by molecular and flow cytometry analysis. This work demonstrates that it is possible to obtain a large-scale production of organoids. We introduced some innovations in the isolation, expansion, and freezing of hPOs from five donors. First of all, the choice of the starting material (islet-depleted pancreas) that allows obtaining a high quantity of hPOs at low passages. On the other hand, we introduced mechanical dissociation and we eliminated the picking step to exclude the operator-depending steps, without affecting the success of the culture ...Continue Reading

References

Mar 2, 2010·Nature Reviews. Drug Discovery·Paul Burn
Apr 13, 2010·Expert Opinion on Drug Metabolism & Toxicology·Qin Meng
Jan 21, 2011·Physiological Reviews·Tom L van BelleMatthias G von Herrath
Feb 7, 2012·PDA Journal of Pharmaceutical Science and Technology·Luis JimenezKasey Travers
Sep 3, 2013·Nature·Madeline A LancasterJuergen A Knoblich
Oct 20, 2014·Nature Medicine·Carey L WatsonMichael A Helmrath
Jan 6, 2015·Cell·Sylvia F BojDavid A Tuveson
Aug 20, 2015·Nature·Dominic GrünAlexander van Oudenaarden
Feb 26, 2016·Nature Cell Biology·Aliya FatehullahNick Barker
Mar 1, 2016·Acta Diabetologica·Silvia PellegriniLorenzo Piemonti
Jan 16, 2019·The EMBO Journal·Norman SachsHans Clevers
Feb 27, 2020·BMC Developmental Biology·Nikitas GeorgakopoulosMeritxell Huch

❮ Previous
Next ❯

Citations

Aug 30, 2020·Nanomaterials·Kyle Bromma, Devika B Chithrani
Mar 5, 2020·Stem Cell Research & Therapy·Joaquim Vives, Laura Batlle-Morera
Jul 28, 2021·Methods : a Companion to Methods in Enzymology·Nguyen-Vi MohamedThomas M Durcan
Jul 29, 2021·Cancer Cell International·Jia YaoXun Li
Aug 6, 2021·Advanced Science·Alessandra DellaquilaTeresa Simon-Yarza
Sep 16, 2021·Islets·Katerina BittenglovaTomas Koblas
Sep 26, 2021·Advanced Materials·Sang Ah YiKi-Bum Lee
Oct 15, 2021·Médecine sciences : M/S·Eléanor LuceJean-Charles Duclos-Vallée

❮ Previous
Next ❯

Methods Mentioned

BETA
biopsies
biopsy
flow cytometry
mechanical dissociation

Software Mentioned

IKAROS
spleen04
FACSDiva
Prism
ImageJ
GraphPad

Related Concepts

Related Feeds

Allogenic & Autologous Therapies

Allogenic therapies are generated in large batches from unrelated donor tissues such as bone marrow. In contrast, autologous therapies are manufactures as a single lot from the patient being treated. Here is the latest research on allogenic and autologous therapies.

Related Papers

Current Diabetes Reports
Jeetindra R A BalakEelco J P de Koning
Stem Cell Investigation
Tohru SugawaraHidenori Akutsu
© 2021 Meta ULC. All rights reserved