Standardized method for the detection of antibodies to aquaporin-4 based on a highly sensitive immunofluorescence assay employing recombinant target antigen

Journal of the Neurological Sciences
Sven JariusKlaus-Peter Wandinger

Abstract

Recently, a highly specific serum autoantibody was discovered in patients with neuromyelitis optica, called NMO-IgG, and aquaporin-4, the most abundant water channel in the CNS, was identified as the target antigen. Several assays for the detection of NMO-IgG/AQP4-Ab have been described. Tests based on recombinant human AQP4 have been repeatedly demonstrated to be more sensitive than the previous gold standard assay, i.e. immunohistochemistry (IHC) on mouse brain tissue. However, the sophisticated techniques applied restrict their availability to few laboratories worldwide. To develop an easy-to-use, recombinant immunofluorescence assay (rIFA) suitable for standardized and high-throughput detection of NMO-IgG/AQP4-Ab. HEK293 cells seeded on cover glasses were transfected with full-length recombinant human AQP4 at large scale. Cover glasses with the immobilized cells were cut into millimetre-sized fragments and transferred to microscopy slides. 151 serum samples from patients with NMO spectrum disorders (NMOSD) and controls were analysed both in the standard IHC assay and in the newly developed rIFA. 25/32 (78.1%) patients with clinically definite NMO and 36/51 (70.6%) of total patients with NMOSD were positive for NMO-IgG/AQP4-...Continue Reading

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Citations

Dec 22, 2010·Der Nervenarzt·C TrebstUNKNOWN Neuromyelitis optica Studiengruppe (NEMOS)
Jan 12, 2013·Der Nervenarzt·B WildemannF Paul
Nov 21, 2013·Journal of Neurology·Marco ZoccaratoLuigi Zuliani
May 28, 2010·Neurological Sciences : Official Journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology·S JariusT Muley
Oct 22, 2013·BMC Neurology·Abhinbhen SarayaThiravat Hemachudha
Jan 17, 2013·Journal of Neuroinflammation·Sven Jarius, Brigitte Wildemann
Jul 19, 2012·PloS One·Felipe von GlehnLeonilda M B Santos
Mar 25, 2014·BMC Neurology·Jason Yongha KimHyoung Doo Shin
Mar 13, 2014·Journal of Neuroinflammation·Sven JariusBrigitte Wildemann
Nov 5, 2013·Multiple Sclerosis International·Felix SchmidtLutz Harms
Oct 19, 2013·Journal of the Neurological Sciences·Joong-Gon ShinHyoung Doo Shin
Dec 13, 2012·Journal of Clinical Neuroscience : Official Journal of the Neurosurgical Society of Australasia·Y LongX Hu
May 17, 2014·Seminars in Immunopathology·Markus Krumbholz, Edgar Meinl
Apr 17, 2015·Multiple Sclerosis and Related Disorders·Christian ProbstLars Komorowski
Feb 20, 2016·Neurology. Neuroimmunology and Neuroinflammation·Imke MetzFriedemann Paul
Dec 6, 2011·Clinical Neurology and Neurosurgery·S JariusB Wildemann
May 31, 2011·Autoimmunity Reviews·Uwe K ZettlRobert Patejdl
May 31, 2011·Autoimmunity Reviews·Andrea T Borchers, M Eric Gershwin
Sep 21, 2010·Journal of the Neurological Sciences·Sven JariusAngela Vincent
Aug 26, 2015·Journal of the Neurological Sciences·S Jarius, B Wildemann
Sep 4, 2015·Journal of the Neurological Sciences·In Hye JeongHo Jin Kim
Mar 29, 2014·Clinical and Experimental Immunology·S JariusF Paul
Nov 26, 2013·Journal of Neurology·Corinna TrebstUNKNOWN Neuromyelitis Optica Study Group (NEMOS)

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