Standardized methods for semen evaluation in a multicenter research study

Journal of Andrology
C BrazilStudy for Future Families Research Group

Abstract

Semen evaluation methodology is complex and difficult to standardize. Rigorously standardized laboratory protocols and strict quality control (QC) are essential for meaningful comparison of data from multiple sites. We describe the methods used for determination of semen volume, sperm concentration, and percent sperm motility in the Study for Future Families, a multicenter study of semen quality in the United States. Each of these 3 semen parameters was assessed using 2 techniques, which provided the opportunity to compare precision and assess suitability for multicenter studies. Detailed protocols were used, and technicians were centrally trained. A total of 509 semen evaluations were performed. Semen volume measured by weight was greater (P <.0001) than that determined by pipetting (3.7 +/- 1.6 mL vs 3.2 +/- 1.6 mL). Sperm concentration determined using hemacytometer chambers was consistently higher (P <.001) than that using disposable MicroCell chambers (81.0 x 10(6)/mL vs 65.9 x 10(6)/mL). Precision was slightly greater for the MicroCell chamber. The percentage of motile sperm was assessed by a simple counting technique as well as by the World Health Organization categorical method that assigns individual motile sperm to "a...Continue Reading

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Citations

Jan 30, 2010·Asian Journal of Andrology·Charlene Brazil
Apr 12, 2013·Human Reproduction Update·David MortimerCharles H Muller
Nov 19, 2013·Journal of Assisted Reproduction and Genetics·J LammersT Fréour
Sep 17, 2016·Human Fertility : Journal of the British Fertility Society·Bryan WoodwardMathew Tomlinson
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May 25, 2010·Environmental Health Perspectives·Jaime MendiolaShanna H Swan
Jul 3, 2013·Asian Journal of Andrology·Renata Walczak-JedrzejowskaJolanta Slowikowska-Hilczer
Jul 18, 2016·Journal of Assisted Reproduction and Genetics·Sandro C Esteves
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Sep 7, 2013·Andrology·J B RedmonUNKNOWN Study for Future Families Research Group
Jan 7, 2017·Reproduction in Domestic Animals = Zuchthygiene·S KargerS P Arlt
Apr 16, 2009·The Journal of Reproduction and Development·Thuy T B VoEui-Bae Jeung
Dec 3, 2010·Fertility and Sterility·Lauren A WiseStacey A Missmer

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