Standardized test for anti-Tr/DNER in patients with paraneoplastic cerebellar degeneration

Neurology. Neuroimmunology and Neuroinflammation
Christian ProbstP Sillevis Smitt

Abstract

To determine sensitivity and specificity of a standardized recombinant cell-based indirect immunofluorescence assay (RC-IFA) for anti-Tr antibodies in comparison to a reference procedure. Delta/Notch-like epidermal growth factor-related receptor (DNER) was expressed in HEK293 and used as a substrate for RC-IFA. HEK293 control cells expressing CDR2/Yo and CDR2L as well as mock-transfected HEK293 cells were used as controls. Serum samples from 38 patients with anti-Tr antibodies (33 with paraneoplastic cerebellar degeneration [PCD] and Hodgkin lymphoma), 66 patients with anti-Tr-negative PCD, 53 patients with Hodgkin lymphoma without neurologic symptoms, 40 patients with rheumatic diseases, and 42 healthy blood donors were tested for anti-DNER reactivity in the RC-IFA. In addition, RC-IFA results were compared to those from a commercial tissue-based IFA using monkey cerebellum. Using the RC-IFA, anti-DNER was detected in all anti-Tr-positive patients but in none of the controls (sensitivity 100%, 95% confidence interval [CI] 92.8%-100%; specificity 100%, 95% CI 98.7%-100%). In comparison, anti-Tr was not detected in 4 samples with low-titer autoantibodies using the commercial tissue-based assay. Preadsorption of sera with either ...Continue Reading

Citations

Jun 16, 2016·Frontiers in Molecular Neuroscience·Marleen H van Coevorden-HameeteCasper C Hoogenraad
Feb 27, 2016·The European Journal of Neuroscience·Aude ChefdevilleVirginie Desestret
Sep 15, 2017·Annals of Clinical and Translational Neurology·Marleen H van Coevorden-HameeteCasper C Hoogenraad
Jun 18, 2017·Frontiers in Immunology·Ralf J LudwigFalk Nimmerjahn
Oct 17, 2017·Neurological Sciences : Official Journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology·Marco ZoccaratoBruno Giometto
Jul 14, 2020·Frontiers in Immunology·Lidia YshiiRoland Liblau

❮ Previous
Next ❯

Datasets Mentioned

BETA
BC035009
BC017503
BC047534

Methods Mentioned

BETA
immunoprecipitation
PCR
transfection
fluorescence microscopy
ELISA

Software Mentioned

SigmaPlot
MedCalc

Related Concepts

Related Feeds

Antiphospholipid Syndrome

Antiphospholipid syndrome or antiphospholipid antibody syndrome (APS or APLS), is an autoimmune, hypercoagulable state caused by the presence of antibodies directed against phospholipids.