PMID: 7939390Jan 1, 1994Paper

Standardizing and reporting results from Mg2+ ISEs, with some notes on sample handling

Scandinavian Journal of Clinical and Laboratory Investigation. Supplementum
N Fogh-Andersen, O Siggaard-Andersen


Mg2+ by ion-selective electrode (ISE) is a direct measure of the reactivity of magnesium ions in plasma, which may be clinically and physiologically more relevant than the concentration of total magnesium. The Mg(2+)-ISE will build up an electric potential which exactly matches the chemical potential of Mg2+ in the sample. Chemical potential (= chemical work per unit of Mg2+) has no absolute value and is difficult to visualize. The results must be standardized, either to the magnesium concentration in a protein-free calibrator or to the concentration of total magnesium in plasma. The constant factor relationship will assure identical clinical discrimination, no matter how the Mg(2+)-ISE results are reported. The general opinion of a conference held in Orlando, Florida in March of 1993 was to define free Mg2+ in plasma as the concentration of magnesium in a saline standard with the same magnesium activity as the sample, and report it in SI units (mmol/L). The small differences in liquid junction potential and water concentration will provide a value for free Mg2+ in plasma of approximately 103% of the true concentration, or 96% of the true molality. Differences between plasma and interstitial fluid and Donnan equilibria across t...Continue Reading

Related Concepts

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.


Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.


Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Clinical Chemistry and Laboratory Medicine : CCLM
Mohammed C Ben RayanaInternational Federation of Clinical Chemistry and Laboratory Medicine (IFCC)
Clinical Chemistry and Laboratory Medicine : CCLM
Mohammed C Ben RayanaIFCC Scientific Division Committee on Point of Care Testing
© 2021 Meta ULC. All rights reserved