Standing Surface Acoustic Wave (SSAW)-Based Fluorescence-Activated Cell Sorter

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Liqiang RenTony Jun Huang

Abstract

Microfluidic fluorescence-activated cell sorters (μFACS) have attracted considerable interest because of their ability to identify and separate cells in inexpensive and biosafe ways. Here a high-performance μFACS is presented by integrating a standing surface acoustic wave (SSAW)-based, 3D cell-focusing unit, an in-plane fluorescent detection unit, and an SSAW-based cell-deflection unit on a single chip. Without using sheath flow or precise flow rate control, the SSAW-based cell-focusing technique can focus cells into a single file at a designated position. The tight focusing of cells enables an in-plane-integrated optical detection system to accurately distinguish individual cells of interest. In the acoustic-based cell-deflection unit, a focused interdigital transducer design is utilized to deflect cells from the focused stream within a minimized area, resulting in a high-throughput sorting ability. Each unit is experimentally characterized, respectively, and the integrated SSAW-based FACS is used to sort mammalian cells (HeLa) at different throughputs. A sorting purity of greater than 90% is achieved at a throughput of 2500 events s-1 . The SSAW-based FACS is efficient, fast, biosafe, biocompatible and has a small footprint,...Continue Reading

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Citations

Feb 1, 2019·Lab on a Chip·Shuaiguo ZhaoTony Jun Huang
Sep 12, 2019·Lab on a Chip·Peiran ZhangTony Jun Huang
Nov 14, 2019·Lab on a Chip·Tianlong ZhangMing Li
Apr 16, 2020·Lab on a Chip·Jinxin ZhangTony Jun Huang
Jun 25, 2019·Microsystems & Nanoengineering·Mengxi WuTony Jun Huang
Jun 13, 2020·Annual Review of Analytical Chemistry·Peiran ZhangTony Jun Huang
May 28, 2019·Advanced Functional Materials·Zhenhua TianTony Jun Huang
Feb 3, 2020·The Journal of the Acoustical Society of America·Jizhou LiuFang Q Hu
Nov 12, 2020·Small·Nanjing HaoTony Jun Huang
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Jun 15, 2021·Lab on a Chip·Erick J Vargas-OrdazVictor J Cadarso
Jul 17, 2021·Lab on a Chip·Kirill KolesnikDavid J Collins
May 8, 2020·Analytical Chemistry·Guoyun SunChia-Hung Chen
Dec 19, 2018·Analytical Chemistry·Peng Li, Tony Jun Huang
Nov 27, 2019·Analytical Chemistry·Ling XiaGongke Li
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Nov 1, 2021·Electrophoresis·Jakub NovotnyThomas Laurell
Nov 23, 2021·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Ce WangXiaodong Wu

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