PMID: 9186782Jan 1, 1997Paper

Staphylococcal protein A binding to VH3 encoded immunoglobulins

International Reviews of Immunology
K N PotterJ D Capra

Abstract

Staphylococcal protein A (SPA) is a B-cell superantigen which binds specifically to the variable region of human VH3 encoded antibodies. We undertook to identify the VH3 regions involved in the interaction with SPA by producing mutant antibodies in the baculovirus expression system. We had previously shown that a single amino acid change at position 57 in the CDR2 of a human SPA nonbinding VH3 encoded rheumatoid factor converted it to an SPA binder, implicating CDR2 in SPA binding. When regions of the mutated binder were exchanged with those from a mouse nonbinding antibody, the pattern of SPA binding indicated that residues in FR1, CDR2 and FR3 are involved in the interaction between VH3 encoded antibodies and SPA. In addition, all three regions are simultaneously required for SPA binding to occur. When any one of the three regions was altered, SPA binding was severely disrupted.

References

Mar 1, 1979·Acta Oto-laryngologica·P PialouxC Fugain
Nov 5, 1992·Journal of Molecular Biology·Z C FanA B Edmundson
Jan 1, 1991·Annual Review of Immunology·A HermanA M Pullen
May 11, 1990·Science·P Marrack, J Kappler
Jun 29, 1990·Science·P LenertM Zanetti
Dec 1, 1985·The Journal of Experimental Medicine·F A NardellaM Mannik
May 2, 1986·European Journal of Biochemistry·T MoksM Uhlén
Feb 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·I Sanz, J D Capra
Sep 1, 1986·The Journal of Clinical Investigation·A I LevinsonM Haidar
Sep 25, 1972·European Journal of Biochemistry·I BjörkJ Sjöquist
Sep 29, 1995·Annals of the New York Academy of Sciences·L M KozlowskiA I Levinson
Jul 1, 1995·The Journal of Clinical Investigation·G J SilvermanM Sasano
Oct 1, 1993·Scandinavian Journal of Immunology·S Ibrahim
Aug 30, 1994·Proceedings of the National Academy of Sciences of the United States of America·L CarayannopoulosJ D Capra
Jan 1, 1994·International Archives of Allergy and Immunology·Z PengF E Simons
Jul 1, 1993·The Journal of Experimental Medicine·J L HillsonE H Sasso
Feb 1, 1993·Scandinavian Journal of Immunology·S IbrahimO Mäkelä
Oct 1, 1991·Current Biology : CB·V Pascual, J D Capra

❮ Previous
Next ❯

Citations

Feb 24, 2000·Molecular Immunology·G GololobovS Paul
Jan 20, 1999·Proceedings of the National Academy of Sciences of the United States of America·A A McCormickR Levy
Sep 20, 2015·Protein Engineering, Design & Selection : PEDS·Mark C JulianPeter M Tessier
Nov 30, 2007·The Journal of Biological Chemistry·Pierre A BarthelemySachdev S Sidhu
Jan 23, 2008·Proceedings·Virginia Pascual, William Clifford Roberts
Feb 22, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Isabelle Bekeredjian-DingGunther Hartmann

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.