PMID: 6452444Apr 1, 1981

Staphylococcus aureus adenosine triphosphatase: inhibitor sensitivity and release from membrane

Journal of Bacteriology
B M Kubak, W W Yotis

Abstract

Homogeneous preparations of cytoplasmic membrane isolated from Staphylococcus aureus 6538P exhibited membrane-associated adenosine triphosphatase (ATPase) activity. Membrane ATPase activity was activated by divalent cations (4.0 mM: Mg2+ greater than Mn2+ greater than Co2+ greater than Zn2+), and ATP was hydrolyzed more readily than other nucleoside triphosphates and phosphorylated substrates. The pH optimum for the membrane ATPase was 6.5. The ATPase could not be released from the membrane by differential osmotic treatments, but detergent treatment effectively solubilized active enzyme. The nonionic detergent Triton X-100 (1%) released a protein with ATPase activity, after substrate-dependent staining in polyacrylamide gels, that differed slightly in electrophoretic migration when compared to the active enzyme solubilized with sodium dodecyl sulfate (0.1%). Membrane-associated ATPase activity was inhibited by N,N'-dicyclohexylcarbodiimide (0.001 to 1 mM) and NaF (50% inhibition at 5 mM NaF). Azide and trypsin inhibited activity, whereas ouabain had a slight inhibitory effect. Diethylstilbestrol showed appreciable activation of the membrane ATPase over the range employed (0.001 to 1 mM).

Citations

Nov 7, 2015·Oncotarget·Maria Claudia VladoiuYves St-Pierre
Nov 30, 2019·Parasite : Journal De La Société Française De Parasitologie·John C Meade

Related Concepts

DNA-dependent ATPase
Azides
Plasma Membrane
Dicyclohexylcarbodiimide
Distilbène
SDS-PAGE
Ouabain
Sodium Chloride, (24)NaCl
Fluoristat
Staphylococcus aureus

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