Staphylococcus aureus Enterotoxin Production in Raw, Holder-Pasteurized, and Ultraviolet-C-Treated Donated Human Milk

Breastfeeding Medicine : the Official Journal of the Academy of Breastfeeding Medicine
Yahya AlmutawifDonna Geddes

Abstract

Background: Some strains of Staphylococcus aureus can produce heat-stable enterotoxins that have been associated with gastritis and potentially necrotizing enterocolitis in preterm infants. Objectives/Hypothesis: To assess the impact of different storage temperatures on S. aureus growth and enterotoxin production in raw, Holder-pasteurized (HP) and ultraviolet-C (UV-C)-treated donated human milk (DHM). Materials and Methods: The milk samples from individual donors were pooled and divided into four equal portions. One portion was HP, the second was UV-C treated, the third was not treated, and the fourth was UV-C treated after being spiked with S. aureus. All samples were incubated at 37°C (18 hours) and 4°C (14 days). Bacterial colony count, enterotoxin A and B, and immune proteins were quantified. Results: At 37°C, the colony count increased in HP DHM and decreased in raw and UV-C-treated DHM. At 4°C, colony counts in HP DHM reduced and were not detected in raw and UV-C-treated DHM from day 8 of incubation. No bacteria were detected in samples that were inoculated before UV-C treatment. Enterotoxin A was only detected in HP-DHM at 37°C from the 9th hour onward. Enterotoxin B was detected in one sample at the 15th hour. I...Continue Reading

References

Oct 28, 1978·British Medical Journal·S A Roberts, M Severn
Jun 1, 1985·Applied and Environmental Microbiology·J C ChangJ D Johnson
Sep 20, 1980·British Medical Journal·B BjörksténO Hernell
Oct 1, 1995·The Journal of Hospital Infection·S E Balmer, A F Williams
Jun 29, 2000·Journal of the American College of Nutrition·P A Ronayne de FerrerJ M Ceriani Cernadas
Feb 19, 2002·Journal of Human Lactation : Official Journal of International Lactation Consultant Association·D B TullyM R Tully
Dec 11, 2003·Revista de saúde pública·Alvaro B SerafiniThiago F N Jubé
Aug 24, 2004·Archives of Disease in Childhood. Fetal and Neonatal Edition·P C LindemannR Lindemann
Sep 1, 2006·Food Microbiology·Hiroshi Fujikawa, Satoshi Morozumi
Jun 6, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Irina V PinchukVictor E Reyes
Oct 5, 2007·Early Human Development·B T HartmannUNKNOWN Australian Neonatal Clinical Care Unit
Sep 29, 2009·The Journal of Pediatrics·Meredith SlutzahRichard J Schanler
Nov 3, 2009·Journal of Pediatric Gastroenterology and Nutrition·C AgostoniUNKNOWN ESPGHAN Committee on Nutrition
Mar 1, 2012·Pediatrics·UNKNOWN Section on Breastfeeding
Jul 11, 2013·PloS One·Lukas ChristenDonna T Geddes
Apr 11, 2015·Archives de pédiatrie : organe officiel de la Sociéte française de pédiatrie·C DewitteV Pierrat
Sep 8, 2016·American Journal of Perinatology·Marzia AlbenzioRosario Magaldi
Oct 19, 2016·Breastfeeding Medicine : the Official Journal of the Academy of Breastfeeding Medicine·Ting MengApril D Fogleman
Feb 6, 2017·Clinics in Perinatology·Jean-Charles Picaud, Rachel Buffin
May 1, 2000·Journal of the Science of Food and Agriculture·Thomas BintsisRichard K Robinson
Mar 1, 2019·Breastfeeding Medicine : the Official Journal of the Academy of Breastfeeding Medicine·Yahya AlmutawifDonna Geddes
Mar 8, 2019·Breastfeeding Medicine : the Official Journal of the Academy of Breastfeeding Medicine·Yahya AlmutawifDonna Geddes

❮ Previous
Next ❯

Citations

Mar 1, 2019·Breastfeeding Medicine : the Official Journal of the Academy of Breastfeeding Medicine·Yahya AlmutawifDonna Geddes
Aug 22, 2020·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Jéssica Bomfim de AlmeidaLucas M Marques

❮ Previous
Next ❯

Methods Mentioned

BETA
enzyme-linked immunosorbent assay

Software Mentioned

ImageJ
R environment
segmented

Related Concepts

Related Feeds

Atrophic Gastritis

Atrophic Gastritis is a process where gastric glandular cells are lost and replaced with firbous tissues, as a result of chronic inflammation. Learn more about Atrophic Gastritis here.

Related Papers

JPEN. Journal of Parenteral and Enteral Nutrition
Sascha Meyer, Ludwig Gortner
Zeitschrift für die gesamte Hygiene und ihre Grenzgebiete
M M AshourA I El-Zayat
The Journal of Pediatrics
R R MoriarteyO A Ulan
Journal of hygiene, epidemiology, microbiology, and immunology
A Osváth-Marton, J Domján
© 2022 Meta ULC. All rights reserved