Staphylococcus aureus penicillin-binding protein 4 and intrinsic beta-lactam resistance.

Antimicrobial Agents and Chemotherapy
U U Henze, B Berger-Bächi

Abstract

Increased levels of production of penicillin-binding protein PBP 4 correlated with in vitro acquired intrinsic beta-lactam resistance in a mutant derived from a susceptible strain of Staphylococcus aureus, strain SG511 Berlin. Truncation of the PBP 4 C-terminal membrane anchor abolished the PBP 4 content of cell membrane preparations as well as the resistance phenotype. A single nucleotide change and a 90-nucleotide deletion, comprising a 14-nucleotide inverted repeat in the noncoding pbp4 gene promoter proximal region, were the only sequence differences between the resistant mutant and the susceptible parent. These mutations were thought to be responsible for the observed overproduction of PBP 4 in the intrinsically beta-lactam-resistant mutant. The pbp4 gene was flanked upstream by the open reading frame abcA, coding for an ATP-binding cassette transporter-like protein showing similarities to eukaryotic multidrug transporters and downstream by a glycerol 3-phosphate cytidyltransferase (tagD)-like open reading frame presumably involved in teichoic acid synthesis. The abcA-pbp4-tagD gene cluster was located in the SmaI-D fragment in the S. aureus 8325 chromosome in close proximity to the RNA polymerase gene rpoB.

References

Jan 1, 1979·Annual Review of Genetics·M Rosenberg, D Court
Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Jan 1, 1991·Annual Review of Microbiology·J M Ghuysen
May 15, 1991·The Biochemical Journal·R P AmblerS G Waley
Dec 1, 1986·European Journal of Clinical Microbiology·B Berger-BächiF H Kayser
Jul 1, 1988·Reviews of Infectious Diseases·B G Spratt, K D Cromie
Dec 1, 1987·Microbiological Reviews·R P Novick
Jun 11, 1986·Nucleic Acids Research·G von Heijne
Jan 15, 1974·Biochemistry·P Y Chou, G D Fasman
Apr 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·J Shine, L Dalgarno
Apr 1, 1983·Journal of Bacteriology·B Berger-Bächi
Jan 1, 1983·Annual Review of Biochemistry·D J Waxman, J L Strominger
Oct 1, 1982·European Journal of Biochemistry·A W WykeM V Hayes
Oct 1, 1981·European Journal of Biochemistry·A W WykeN A Curtis
Oct 1, 1994·Trends in Microbiology·B Berger-Bächi
Jul 1, 1994·The Biochemical Journal·H F ChambersC J Hackbarth

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Citations

Feb 1, 1997·International Journal of Antimicrobial Agents·E VasilievaB Berger-Bächi
May 18, 2000·Microbes and Infection·T L Smith, W R Jarvis
Mar 6, 2004·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Yuki KatayamaHenry F Chambers
Feb 13, 2001·Antimicrobial Agents and Chemotherapy·G Schrader-Fischer, B Berger-Bächi
Mar 21, 2006·Journal of Bacteriology·Angelika Gründling, Olaf Schneewind
Jul 22, 2011·Annals of Clinical Microbiology and Antimicrobials·James T RiordanJohn E Gustafson
Feb 6, 2008·FEMS Microbiology Reviews·André ZapunThierry Vernet
Jun 3, 2015·Journal of the American Chemical Society·Samir GautamDavid A Spiegel
Mar 15, 2015·Journal of Microbiological Methods·Benoit MantionMarie-Françoise Prère
Jul 27, 2002·Annual Review of Microbiology·Timothy R Walsh, Robin A Howe
Aug 1, 1996·FEMS Microbiology Letters·S Píriz DuránB Berger-Bächi
Jun 23, 2018·Microorganisms·Thaina M da CostaSom S Chatterjee
Apr 5, 2017·Antimicrobial Agents and Chemotherapy·Stephanie M HamiltonHenry F Chambers
May 23, 2014·Journal of Clinical Microbiology·Eileen M BurdTimothy D Read
Apr 14, 2016·Antimicrobial Agents and Chemotherapy·Liana C ChanSom S Chatterjee
Oct 20, 2020·Expert Review of Anti-infective Therapy·Juan Alberto Guevara SalazarJosé Guadalupe Trujillo Ferrara
Jun 6, 1998·Journal of Bacteriology·A Wada, H Watanabe
Dec 1, 1996·Microbiological Reviews·I T PaulsenR A Skurray
Sep 1, 1996·Antimicrobial Agents and Chemotherapy·U U Henze, B Berger-Bächi

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