Staphylococcus aureus PhoU Homologs Regulate Persister Formation and Virulence

Frontiers in Microbiology
Yongpeng ShangDi Qu

Abstract

PhoU homologs are one of the determinant factors in the regulation of persister formation and phosphate metabolism in many bacterial species; however, the functions of PhoU homologs exhibit species-specific characteristics. The pathogenesis of Staphylococcus aureus is closely correlated with persister formation and virulence factors. The functions of two PhoU homologs, PhoU1 and PhoU2, in S. aureus are unclear yet. In this study, single- and double-deletion mutants of phoU1 and phoU2 were generated in strain USA500 2395. The ΔphoU1 or ΔphoU2 mutants displayed a change in persister formation and virulence compared to the parent strain; the persisters to vancomycin and levofloxacin were decreased at least 1,000-fold, and the number of intracellular bacteria surviving in the A549 cells for 24 h decreased to 82 or 85%. The α-hemolysin expression and activity were increased in the ΔphoU2 mutants. Transcriptome analysis revealed that 573 or 285 genes were differentially expressed by at least 2.0-fold in the ΔphoU1 or ΔphoU2 mutant vs. the wild type. Genes involved in carbon and pyruvate metabolism were up-regulated, and virulence genes and virulence regulatory genes were down-regulated, including type VII secretion system, serine pro...Continue Reading

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Citations

May 22, 2021·ACS Infectious Diseases·Jinxin ZhengZhijian Yu
Oct 21, 2021·Journal of Medical Microbiology·Hongyan WangYongpeng Shang

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Datasets Mentioned

BETA
GSE139071
CP007499

Methods Mentioned

BETA
gene knockout
reverse transcription-PCR
PCR
Assay
RNA-Seq

Software Mentioned

DEGseq
Cytoscape
BED Tools
SPSS
Bowtie2
IPA

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