PMID: 2492591Feb 1, 1989Paper

Staphylococcus aureus tetrapeptide with high chemotactic potency and efficacy for human leukocytes

Journal of Leukocyte Biology
A RotE J Leonard

Abstract

A chemotactic tetrapeptide from culture fluids of Staphylococcus aureus was purified to homogeneity by reverse-phase high-pressure liquid chromatography. The peptide comprises equimolar methionine, leucine, isoleucine, and phenylalanine. It inhibited binding of fluoresceinated fMet-Leu-Phe-Lys to human monocytes, which showed that it interacted with the formyl-methionyl peptide receptor and suggested that it was a formyl-methionyl peptide. Based on a comparison of dose-response curves for inhibition of fluoresceinated fMet-Leu-Phe-Lys binding, the relative affinity of the peptide for the receptor was comparable to that of fMet-Leu-Phe-Lys. At optimal concentrations, chemotactic efficacy (percentage of monocytes migrating to the attractant) was 53 +/- 4%, in contrast to 36 +/- 3% for the reference attractant fMet-Leu-Phe. Since approximately 60% of human monocytes have formyl-peptide receptors, the bacterial peptide is capable of attracting all receptor-bearing monocytes.

Citations

Sep 19, 2000·Infection and Immunity·K E VeldkampK P van Kessel
Jul 16, 2005·Clinical Microbiology Reviews·Bénédicte Fournier, Dana J Philpott
Jul 9, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Erica L SouthgateRichard D Ye
Jun 8, 2002·FEMS Immunology and Medical Microbiology·Savita P RaoAntonino Catanzaro
Jun 22, 1999·Infection and Immunity·M C CalleganM S Gilmore
Jun 17, 2000·Clinics in Dermatology·J M Schröder
Nov 1, 1994·Journal of Pharmacological and Toxicological Methods·F GuessousM Roch-Arveiller
Sep 1, 1993·Developmental and Comparative Immunology·H Schneeweiss, L Renwrantz
Jul 13, 2017·Journal of Medicinal Chemistry·Sarah Line SkovbakkeHenrik Franzyk

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