STAQ: A route toward low power, multicolor nanoscopy

Microscopy Research and Technique
Tilman RosalesJay R Knutson

Abstract

Nanoscopy has now become a real procedure in fluorescence microscopy of living cells. The STED/RESOLFT family of nanoscopy approaches has the best prospects for delivering high speed imaging, but the history of STED includes a continuing struggle to reduce the deactivation power applied, along with difficulties in achieving simultaneous multicolor images. In this manuscript, we present a concept for a similar real-time nanoscopy, using a new class of bipartite probes that separate the luminescent and quenching functions into two coupled molecules. In particular, the STAQ (Superresolution via Transiently Activated Quencher) example we show herein employs the excited state absorbance (not ground state) of the partner to accept energy from and quench the luminescent dye. The result is that much less deactivation power is needed for superresolved (∼50 nm) imaging. Moreover, the TAQ partner excited by the "donut" beam is shown to quench several different visible dyes via the same mechanism, opening the door to easier multicolor imaging. We demonstrate three dyes sharing the same deactivation and show examples of superresolved multicolor images. We suggest STAQ will facilitate the growth of real-time nanoscopy by reducing confounding...Continue Reading

References

Oct 1, 1996·Protein Science : a Publication of the Protein Society·J WolffL Knipling
Apr 28, 2001·Biophysical Journal·V BarzdaL Valkunas
Dec 7, 2002·Nature Biotechnology·Antje KepplerKai Johnsson
Oct 30, 2003·Journal of the American Chemical Society·Mircea CotletFrans C De Schryver
Oct 7, 2004·Current Opinion in Neurobiology·Stefan W HellStefan Jakobs
Dec 1, 2005·Chemical Communications : Chem Comm·Zongren Zhang, Samuel Achilefu
Aug 10, 2006·Nature Methods·Michael J RustXiaowei Zhuang
Aug 12, 2006·Science·Eric BetzigHarald F Hess
Sep 19, 2006·Biophysical Journal·Samuel T HessMichael D Mason
Oct 19, 2007·Proceedings of the National Academy of Sciences of the United States of America·Jeremy M BaskinCarolyn R Bertozzi
Feb 23, 2008·Science·Volker WestphalStefan W Hell
Jan 13, 2009·Biophysical Journal·Jan SchröderGeorgyi V Los
Jun 16, 2010·ACS Chemical Biology·Abhijit BanerjeeSusan Bane
Mar 4, 2011·Optics Express·Johanna BückersStefan W Hell
Mar 4, 2011·Optics Express·Jeffrey R MoffittJens Michaelis
Jun 7, 2011·Nature Methods·Giuseppe VicidominiStefan W Hell
Jun 22, 2011·Biophysical Journal·Brian R RankinStefan W Hell
Jul 27, 2011·Nano Letters·Katrin I WilligStefan W Hell
Nov 22, 2011·Biophysical Journal·Jan TønnesenU Valentin Nägerl
Mar 29, 2012·Optics Express·Silvia GalianiPaolo Bianchini
Feb 8, 2013·Optics Express·Emiliano RonzittiAlberto Diaspro
Jul 9, 2013·Nature Methods·Andriy ChmyrovStefan W Hell
Mar 19, 2014·Journal of Biophotonics·Ivàn Coto HernàndezGiuseppe Vicidomini
Mar 26, 2014·Optics Express·Bin YangBrahim Lounis
Jul 2, 2014·Current Opinion in Chemical Biology·Hans Blom, Jerker Widengren
Apr 4, 2015·Optics Express·Fabian BergermannStefan W Hell

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Citations

Nov 6, 2021·Nanoscale·Alan M SzalaiFernando D Stefani

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