START-GAP3/DLC3 is a GAP for RhoA and Cdc42 and is localized in focal adhesions regulating cell morphology

Biochemical and Biophysical Research Communications
Katsuhisa KawaiH Yagisawa

Abstract

In the human genome there are three genes encoding RhoGAPs that contain the START (steroidogenic acute regulatory protein (StAR)-related lipid transfer)-domain. START-GAP3/DLC3 is a tumor suppressor gene similar to two other human START-GAPs known as DLC1 or DLC2. Although expression of START-GAP3/DLC3 inhibits the proliferation of cancer cells, its molecular function is not well understood. In this study we carried out biochemical characterization of START-GAP3/DLC3, and explored the effects of its expression on cell morphology and intracellular localization. We found that START-GAP3/DLC3 serves as a stimulator of PLCdelta1 and as a GAP for both RhoA and Cdc42 in vitro. Moreover, we found that the GAP activity is responsible for morphological changes. The intracellular localization of endogenous START-GAP3/DLC3 was explored by immunocytochemistry and was revealed in focal adhesions. These results indicate that START-GAP3/DLC3 has characteristics similar to other START-GAPs and the START-GAP family seems to share common characteristics.

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Citations

Sep 13, 2008·The Journal of Biological Chemistry·Tai Young KimRudy L Juliano
Apr 17, 2009·Journal of Gastroenterology and Hepatology·Judy Wai Ping YamIrene Oi-Lin Ng
Sep 15, 2009·PloS One·Pooneh MokarramHassan Ashktorab
Oct 4, 2011·The Journal of Endocrinology·Barbara J Clark
Jul 26, 2011·European Journal of Medical Genetics·Florence PetitSylvie Manouvrier-Hanu
Jan 28, 2009·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Katsuhisa KawaiHitoshi Yagisawa
Oct 12, 2013·International Journal of Cancer. Journal International Du Cancer·Frankie Chi Fat Ko, Judy Wai Ping Yam
Oct 31, 2009·Liver International : Official Journal of the International Association for the Study of the Liver·Frankie Chi Fat KoJudy Wai Ping Yam
Aug 21, 2010·Liver International : Official Journal of the International Association for the Study of the Liver·Carmen Chak-Lui WongIrene Oi-Lin Ng
Oct 1, 2013·Biochimie·Fabien Alpy, Catherine Tomasetto
Jan 16, 2013·Journal of Receptor and Signal Transduction Research·Sally El-Sitt, Mirvat El-Sibai
Nov 10, 2009·Advances in Enzyme Regulation·Katsuhisa KawaiHitoshi Yagisawa
Mar 3, 2009·Biochemical and Biophysical Research Communications·Katsuhisa KawaiHitoshi Yagisawa
Aug 10, 2016·Journal of Cell Science·Janina HendrickMonilola A Olayioye
Feb 26, 2009·Médecine sciences : M/S·Fabien AlpyCatherine Tomasetto
Nov 17, 2016·Small GTPases·Janina Hendrick, Monilola A Olayioye
Apr 10, 2021·International Journal of Biological Macromolecules·Guanghui Ren, Guorong Li
Feb 13, 2015·Journal of Cell Science·Anja C BraunMonilola A Olayioye
Feb 26, 2009·Genes & Development·Kyle R LegateReinhard Fässler

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