PMID: 9162112May 1, 1997Paper

Starvation for a specific amino acid induces high frequencies of rho- mutants in Saccharomyces cerevisiae

Current Genetics
Erich Heidenreich, Ulrike Wintersberger

Abstract

Auxotrophic yeast cells were starved on solid media for their respective essential amino acid in the course of "adaptive mutation" experiments. Thereby, high proportions of mitochondrially respiratory deficient (rho-) mutants accumulated among the cells stressed on selective plates. Using a strain with a plus-four frameshift mutation in a chromosomal gene involved in lysine biosynthesis, we observed that many of the revertant colonies which arose late under the selective pressure were composed of mixtures of rho+ and rho- cells, indicating that they originated from founder cells containing intact as well as defective mitochondrial genomes. We show that in spite of the slower growth of rho- cells the late-appearing colonies cannot be interpreted as descending from rho- revertants present before selective plating.

Citations

Jul 27, 2001·Mutation Research·W P Angerer
Feb 20, 1998·Current Opinion in Genetics & Development·S M Rosenberg
Apr 1, 1997·European Journal of Biochemistry·K R BirikhF Eckstein
Oct 12, 2004·Experimental Cell Research·Herfried EislerErich Heidenreich
Jan 15, 2017·FEMS Yeast Research·Serge Gangloff, Benoit Arcangioli
Jul 4, 2001·Nature Reviews. Genetics·S M Rosenberg
Aug 10, 2007·Critical Reviews in Biochemistry and Molecular Biology·Erich Heidenreich

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