STAT-5 is activated constitutively in T cells, B cells and monocytes from patients with primary Sjögren's syndrome

Clinical and Experimental Immunology
M PertovaaraP Isomäki

Abstract

The expression and phosphorylation of signal transducer and activator of transcription-1 (STAT-1) have been shown to be markedly increased in the salivary gland epithelial cells of patients with primary Sjögren's syndrome (pSS). The present aim was to investigate the activation status of different STAT proteins in peripheral blood (PB) lymphocytes and monocytes, and their correlations with clinical parameters in patients with pSS. To this end, PB samples were drawn from 16 patients with active pSS and 16 healthy blood donors, and the phosphorylation of STAT-1, -3, -4, -5 and -6 proteins was studied in T cells, B cells and monocytes using multi-colour flow cytometry. In addition, mRNA expression of STAT molecules in PB mononuclear cells (PBMC) was studied with quantitative reverse transcriptase-polymerase chain reaction (RT-PCR). Basal phosphorylation of STAT-5 was found to be significantly higher in PB T cells, B cells and monocytes in patients with pSS than in healthy controls. The expression of STAT-5 mRNA was not increased in PBMC. pSTAT-5 levels in B cells and monocytes showed a significant correlation with serum immunoglobulin (Ig)G levels and anti-SSB antibody titres. Constitutive STAT-5 activation in monocytes and CD4(+)...Continue Reading

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Citations

Mar 9, 2018·Frontiers in Immunology·Rob J W ArtsMihai G Netea
Apr 27, 2019·Frontiers in Immunology·Michael BitarUlrich Sack
Apr 3, 2019·Frontiers in Immunology·Andri L LemarquisBjorn R Ludviksson
Jun 24, 2017·Journal of Ophthalmology·Hua WangQiang-Xiang Li
May 22, 2017·Clinical Reviews in Allergy & Immunology·María MorellConcepción Marañón

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