PMID: 15240699Jul 9, 2004Paper

STAT1 regulates lipopolysaccharide- and TNF-alpha-dependent expression of transporter associated with antigen processing 1 and low molecular mass polypeptide 2 genes in macrophages by distinct mechanisms

The Journal of Immunology : Official Journal of the American Association of Immunologists
L MarquésA Celada

Abstract

Transporter associated with Ag processing 1 and low molecular mass polypeptide 2 (LMP2) are essential for class I MHC function and share a common bidirectional promoter. In murine bone marrow-derived macrophages, LPS and TNF-alpha induced Tap1 and up-regulated Lmp2, which is constitutively expressed at low levels. These two genes are induced by LPS and TNF-alpha with distinct kinetics, at 6 and 12-24 h, respectively. Using macrophages derived from the TNF-alpha receptors of knockout mice, we found that induction by LPS is not due to the autocrine production of TNF-alpha. In macrophages from STAT-1 knockout mice, neither LPS nor TNF-alpha induced the expression of Tap1 or Lmp2. The shared promoter contains several areas that can be controlled by STAT-1, such as the proximal and distal IFN-gamma activation site (GAS) boxes in the direction of the Tap1 gene. By making deletions of the promoter, we determined that only the proximal GAS box is required for LPS induction of Tap1 and Lmp2. In contrast, TNF-alpha induction of these two genes is dependent on the IFN regulatory factor-1 and NF-kappaB boxes, and not on the GAS box. Our experiments using gel shift analysis and Abs indicated that STAT1 binds to the GAS box in nuclear extrac...Continue Reading

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Citations

Aug 16, 2006·European Journal of Immunology·Ester Sànchez-TillóAntonio Celada
Jan 24, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Chengjiang GaoPaul C Kuo
Jan 10, 2012·Nature Reviews. Immunology·José M González-NavajasEyal Raz
Aug 7, 2019·Thorax·James Andrew CoultasPeter J Openshaw
May 4, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Andrée YeramianAntonio Celada
Apr 7, 2005·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Pascale KropfIngrid Müller
May 20, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Ester Sánchez-TillóAntonio Celada
Mar 21, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Annabel F ValledorAntonio Celada
May 17, 2006·European Journal of Immunology·Andrée YeramianAntonio Celada
Jan 5, 2020·BMC Research Notes·Abhishikta BasuNaoya Kenmochi
May 4, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Cristina CasalsAntonio Celada
May 20, 2008·Cancer Immunology, Immunotherapy : CII·Lazar VujanovicLisa H Butterfield
Jan 9, 2009·European Journal of Immunology·Luis ArpaAntonio Celada
Sep 8, 2019·The Journal of Immunology : Official Journal of the American Association of Immunologists·Robert R HainesJeremy M Boss
Apr 10, 2020·European Journal of Immunology·Lorena Valverde-EstrellaAntonio Celada
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