PMID: 8938718Jan 1, 1996Paper

State-dependent modulation of mSlo, a cloned calcium-dependent potassium channel

Neuropharmacology
M MüllerI B Levitan

Abstract

The mouse slopoke calcium-dependent potassium channel (mSlo) has been expressed heterologously in COS cells, and incorporated from COS cell membranes into artificial phospholipid bilayers. Under control conditions, the channel is not modulated by ATP. However, when mSlo is treated first with the calcium-dependent potassium channel opener NS004, which itself increases the open probability of the channel, subsequent addition of ATP causes a large further increase in channel open probability. An increase in channel activity is not by itself sufficient to confer sensitivity to ATP, because ATP does not modulate channels whose open probability has been increased by elevated calcium or depolarized voltage. The ATP analog AMP-PNP has only minimal effects on channel activity after treatment with NS004, suggesting that hydrolysis of the ATP is required for its action on mSlo. A peptide inhibitor of the calcium/calmodulin-dependent protein kinase II (CaMKII) blocks the modulation of mSlo by ATP, whereas peptide inhibitors of other serine/threonine protein kinases are without effect. The results are consistent with a state-dependent modulation of mSlo by ATP, possibly via phosphorylation.

References

Apr 1, 1991·Neuron·I NishimotoP Gardner
Sep 1, 1990·Trends in Biochemical Sciences·B E Kemp, R B Pearson
Jan 1, 1994·Annual Review of Physiology·I B Levitan
May 10, 1995·Annals of the New York Academy of Sciences·S L SwopeR L Huganir
Apr 1, 1993·Trends in Neurosciences·L A RaymondR L Huganir
Nov 1, 1994·Brain Research. Molecular Brain Research·S I DworetzkyV K Gribkoff
Dec 20, 1993·Annals of the New York Academy of Sciences·W A Catterall

❮ Previous
Next ❯

Related Concepts

Related Feeds

Anthelmintics

Anthelmintics or antihelminthics are a group of antiparasitic drugs that expel parasitic worms (helminths) and other internal parasites from the body by either stunning or killing them and without causing significant damage to the host. Discover the latest research on anthelmintics here.