PMID: 6975374Jan 1, 1980Paper

State modifications of thymocyte plasma membrane proteins and lipids by mitogenic doses of concanavalin A: a Raman study on isolated membrane vesicles

Journal of Receptor Research
S P VermaD F Wallach

Abstract

Sealed plasma membrane vesicles from rabbit thymocytes were reacted with 0.4-10 micrograms concanavalin A/ml, that is at concentrations that produce cooperative lectin-binding in vivo and in vitro and induce mitogenesis of intact cells. The effects of concanavalin A were monitored by laser Raman spectroscopy of the vesicles in the CH-stretching region. This technique revealed moderately cooperative lipid state transitions in untreated membranes centered at about -6 degrees and 25 degrees, as well as a protein state change at about 43 degrees C. Concanavalin A treatment of the membranes lowered the transition temperatures of the integral of 25 degrees an integral of 43 degrees state changes indicating a direct effect of lectin binding on membrane protein/lipid organization. It is proposed that the primary protein involved is the 55,000D transmembrane protein (Schmidt-Ullrich, R., Mikkelsen, R. B. and Wallach, D. F. H. (1978), J. Biol. Chem. 253, 6973-6978), known to be the high-affinity receptor for concanavalin A, and that the concanavalin A-sensitive integral of 25 degrees transition arises from lipids associated with this protein.

References

Nov 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·R B MikkelsenD F Wallach
Jan 1, 1977·Methods in Cell Biology·D F Wallach, R Schmidt-Ullrich
Jan 24, 1977·Biochemical and Biophysical Research Communications·S P Verma, D F Wallach
Apr 19, 1976·Biochemical and Biophysical Research Communications·R Schmidt-Tllrich, D F Wallach
May 25, 1977·Biochimica Et Biophysica Acta·M R Bunow, I W Levin
Dec 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·H Borochov, M Shinitzky
Apr 5, 1976·Biochimica Et Biophysica Acta·S P Verma, D F Wallach
Aug 9, 1974·Biochimica Et Biophysica Acta·R Schmidt-UllrichE Ferber
Mar 1, 1977·Biochimica Et Biophysica Acta·B P Gaber, W L Peticolas

❮ Previous
Next ❯

Citations


❮ Previous
Next ❯

Related Concepts

Related Feeds

Autoimmune Polyendocrine Syndromes

This feed focuses on a rare genetic condition called Autoimmune Polyendocrine Syndromes, which are characterized by autoantibodies against multiple endocrine organs. This can lead to Type I Diabetes.

Autoimmune Polyendocrinopathies

Autoimmune polyendocrinopathies, also called polyglandular autoimmune syndromes (PGASs), or polyendocrine autoimmune syndromes(PASs), are a heterogeneous group of rare diseases characterized by autoimmune activity against more than one endocrine organ, although non-endocrine organs can be affected. Discover the latest research on autoimmune polyendocrinopathies here.