State-of-the-art of the hyphenation of capillary electrochromatography with mass spectrometry

Electrophoresis
Elena Barceló-BarrachinaM Teresa Galceran

Abstract

The high separation efficiency and loading capacity of capillary electrochromatography (CEC) make it an attractive separation mode for coupling with mass spectrometry (MS), which has the ability to unambiguously identify analytes with high selectivity and sensitivity. We present an overview of recent advances on both instrumentation and separation columns employed in CEC-MS systems. In particular, the main characteristics of the stationary phases, as well as the configurations of the column outlet that are related with the coupling arrangements of the MS ionization sources, are reported. At present, packed columns and conventional electrospray ionization (ESI) sources are mainly employed in CEC-MS. Nevertheless, the use of monolithic capillary columns and nanoelectrospray sources has the potential for wide acceptance in the next future. Moreover, the main features of several mass analyzers including ion trap, quadrupole, time-of-flight, magnetic sector, and Fourier transform-ion cyclotron resonance are examined. Finally, current applications of this technology, mainly in the pharmaceutical field and proteomics, are reviewed.

Citations

Jun 17, 2010·Se pu = Chinese journal of chromatography·Zian LinGuonan Chen
Apr 14, 2015·Free Radical Research·H StutzP M Eckl
Jan 27, 2005·Journal of Mass Spectrometry : JMS
Dec 1, 2009·Journal of Chromatography. a·Giovanni D'Orazio, Salvatore Fanali
Oct 28, 2005·Electrophoresis·Jens OhnesorgeHermann Wätzig
Mar 15, 2005·Electrophoresis·David C Simpson, Richard D Smith
Oct 18, 2005·Journal of Separation Science·Francisco MerinoDolores Pérez-Bendito
Nov 1, 2006·Electrophoresis·Dean NortonShahab A Shamsi
May 1, 2007·Electrophoresis·Elena Barceló-BarrachinaMaria Teresa Galceran
Mar 9, 2010·Electrophoresis·Zeineb AturkiSalvatore Fanali
May 10, 2016·Journal of Pharmaceutical and Biomedical Analysis·Sven DeclerckDebby Mangelings
Apr 24, 2007·Electrophoresis·Heimo ScherzGünther K Bonn
Jul 15, 2005·Journal of Separation Science·Ana Juan-GarcíaYolanda Picó
Nov 30, 2005·Electrophoresis·Ewa Dabek-Zlotorzynska, Valbona Celo
Nov 30, 2005·Electrophoresis·Sebastiaan Eeltink, Wim Th Kok
Jun 12, 2009·Electrophoresis·Frantisek Svec
Nov 16, 2007·Analytical Chemistry·Dean Norton, Shahab A Shamsi
Jun 15, 2005·Analytical Chemistry·R K Gilpin, L A Pachla
Nov 1, 2005·Analytical Chemistry·Dean NortonShahab A Shamsi

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.