State-of-the-art protein engineering approaches using biological macromolecules: A review from immobilization to implementation view point

International Journal of Biological Macromolecules
Muhammad BilalXuehong Zhang

Abstract

Over the past years, technological and scientific advances have proven biocatalysis as a sustainable alternative than traditional chemical catalysis including organo- or metallocatalysis. In this context, immobilization based approaches represent simple but effective routes for engineering enzyme catalysts with higher activities than wild-type derivatives. Many enzymes including oxidoreductases have been engineered by rational and directed evolution, to realize the catalytic activity, enantioselectivity, and stability attributes which are essential for their biotechnological exploitation. Induce yet stable activity in enzyme catalysis offer new insights and motivation to engineer efficient catalysts for practical and commercial purposes. It has now become possible to envisage substrate accessibility to the catalytic site of the enzyme by current computational capabilities that reduce the experimental work related to the enzyme selection, screening, and engineering. Herein, state-of-the-art protein engineering approaches for improving enzymatic activities including chemical modification, directed evolution, and rational design or their combination methods are discussed. The emphasis is also given to the applications of the resul...Continue Reading

Citations

Nov 6, 2018·Critical Reviews in Biotechnology·Muhammad BilalHafiz M N Iqbal
Feb 20, 2019·Preparative Biochemistry & Biotechnology·Luane Ferreira GarciaMariângela Fontes Santiago
Jun 19, 2019·Critical Reviews in Food Science and Nutrition·Muhammad Bilal, Hafiz M N Iqbal
Jul 1, 2020·Expert Opinion on Biological Therapy·Saptashwa DattaHatem Zayed
Feb 3, 2019·Zeitschrift Für Naturforschung. C, a Journal of Biosciences·Angela Viviana Ruales-SalcedoJohn M Woodley
Sep 27, 2018·International Journal of Molecular Sciences·Gerardo ZambranoAngela Lombardi
Jan 17, 2021·International Journal of Biological Macromolecules·Soudabeh FarhadiMojtaba Mortazavi
Jan 20, 2021·Biotechnology and Applied Biochemistry·Gerardo ZambranoFlavia Nastri
Jan 30, 2021·Frontiers in Pharmacology·Ziyu WangDianwen Ju
May 3, 2021·Food Chemistry·Natália Carminatti RicardiTania Maria Haas Costa
Jan 21, 2019·Environment International·Muhammad BilalHafiz M N Iqbal
May 24, 2021·Advances in Colloid and Interface Science·Muhammad BilalHafiz M N Iqbal
Jun 15, 2021·International Journal of Biological Macromolecules·Bruna Coelho de AndradeClaucia Fernanda Volken de Souza
Aug 30, 2021·Biotechnology Advances·Rafael C RodriguesRoberto Fernandez-Lafuente
Sep 11, 2021·International Journal of Biological Macromolecules·Shuai LiuHafiz M N Iqbal
Dec 3, 2021·Biomacromolecules·Justin M Horn, Allie C Obermeyer

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