Stathmokinetic analysis of granulosa cell proliferation in antral follicles of cyclic rats

Biology of Reproduction
A N Hirshfield

Abstract

On Day 1 of the estrous cycle (estrus), most of the largest follicles in the ovaries of rats appear to be healthy. By the following day, however, nearly one-third of all antral follicles show clear signs of atresia. The remaining healthy follicles are presumably those which will go on to ovulate. The object of this study was to distinguish between two possibilities: 1) that all of the largest follicles in the ovary on Day 1 subsequently undergo atresia on Day 2, and a new cohort of healthy follicles arises to replace them, or 2) that one single cohort of follicles on Day 1 is the source of both the healthy and atretic follicles seen on Day 2. For this purpose, the rate of granulosa cell proliferation was determined on each day of the 4-day estrous cycle using a metaphase arrest technique. Follicles grew most slowly on Day 4; small follicles (181-646 cells in the largest cross section) had a mitotic index (MI) of 8.1% after a 4-h block. In contrast, on Day 1 granulosa cells were proliferating rapidly; small follicles had an MI of 15.9%. This high rate of cell proliferation was also characteristic of large follicles. Estimated growth rates predict that, in healthy follicles, the number of granulosa cells should have almost double...Continue Reading

Citations

Sep 1, 1994·Biulleten' eksperimental'noĭ biologii i meditsiny·T G Borovaia, O V Volkova
Oct 1, 1989·Journal of Steroid Biochemistry·L J Spicer, J M Hammond
Nov 24, 1999·Reproductive Toxicology·W J HrusheskyP A Wood
Jun 11, 2004·Biology of Reproduction·R S JaiswalG P Adams
Feb 3, 2006·Biology of Reproduction·John J Peluso
Apr 4, 2012·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Gloria E HoffmanKaren Usdin
Feb 12, 2014·Reproduction : the Official Journal of the Society for the Study of Fertility·John J Peluso, James K Pru
Nov 7, 2006·Molecular and Cellular Endocrinology·Jennifer D CannonCharles L Chaffin
Mar 23, 2017·The Journal of Reproduction and Development·Kouji Komatsu, Satoru Masubuchi
Jun 7, 2007·Molecular Reproduction and Development·Chang Joo LeeYong-Dal Yoon

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