Statin pleiotropy prevents rho kinase-mediated intestinal epithelial barrier compromise induced by Blastocystis cysteine proteases

Cellular Microbiology
Haris MirzaKevin S W Tan

Abstract

Blastocystis is an enteric parasite that causes acute and chronic intestinal infections, often non-responsive to conventional antibiotics. The effects of Blastocystis infections on human epithelial permeability are not known, and molecular mechanisms of Blastocystis-induced intestinal pathology remain unclear. This study was conducted to determine whether Blastocystis species alters human intestinal epithelial permeability, to assess whether these abnormalities are rho kinase (ROCK)-dependent, and to investigate the therapeutic potential of the HMG-CoA reductase inhibitor Simvastatin in altered intestinal epithelial barrier function. The effect of metronidazole resistant (Mz(r)) Blastocystis isolated from a symptomatic patient on human colonic epithelial monolayers (Caco-2) was assessed. Modulation of enterocyte myosin light chain phosphorylation, transepithelial fluorescein isothiocyanate-dextran fluxes, transepithelial resistance, cytoskeletal F-actin and tight junctional zonula occludens-1 (ZO-1) by parasite cysteine proteases were measured in the presence or absence of HMG-CoA reductase and ROCK inhibition. Blastocystis significantly decreased transepithelial resistance, increased epithelial permeability, phosphorylated myo...Continue Reading

References

Jun 1, 1996·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·M AnderssonM Bakhiet
Jul 7, 1999·The Journal of Clinical Investigation·H WanC Robinson
Jan 8, 2000·Journal of Pediatric Surgery·S RokhsarJ D Phillips
Jan 5, 2002·Acta Tropica·Hande DagciSeza Budak
Jun 5, 2003·Nature Reviews. Molecular Cell Biology·Kirsi Riento, Anne J Ridley
Sep 2, 2004·Acta Dermato-venereologica·Rossano ValsecchiVincenzo Greco
Nov 13, 2004·The Journal of Allergy and Clinical Immunology·Charles E Reed, Hirohito Kita
Sep 10, 2005·Parasitology Research·Manoj K PuthiaKevin S W Tan
Mar 7, 2006·Parasitology Research·Selena W S SioKevin S W Tan
Jun 7, 2006·Current Opinion in Cardiology·Kunal SarkarJawahar L Mehta
Jan 24, 2007·Trends in Parasitology·C Rune StensvoldC Graham Clark
Dec 8, 2007·Parasitology Research·Kenneth H S WongKevin S W Tan
Dec 11, 2007·Trends in Molecular Medicine·Chao-Yung WangJames K Liao
Apr 19, 2008·The Journal of Infectious Diseases·Zacharie TaoufiqDominique Mazier
Jul 29, 2008·American Journal of Physiology. Lung Cellular and Molecular Physiology·Weiguo ChenJeffrey R Jacobson
Oct 11, 2008·Parasitology Research·Haris Mirza, Kevin S W Tan
Jan 27, 2009·Atherosclerosis·Anju NohriaPeter Ganz
Feb 10, 2009·Current Pharmaceutical Design·Qian Zhou, James K Liao
Mar 31, 2009·Transactions of the Royal Society of Tropical Medicine and Hygiene·A KurniawanH V Smith
May 2, 2009·Cardiovascular Research·Cristina RodríguezLina Badimon
Jul 8, 2009·International Journal for Parasitology·Naveed Ahmed Khan, Ruqaiyyah Siddiqui
Jul 17, 2009·Molecular Biology of the Cell·Christina M Van ItallieJames M Anderson
Oct 17, 2009·Journal of Clinical Gastroenterology·Christen R StensvoldRebecca J Traub
Oct 27, 2009·Nature Reviews. Immunology·Jerrold R Turner
Feb 19, 2010·Parasitology Research·Samudi ChandramathiUmah Rani Kuppusamy
Apr 7, 2010·Parasitology International·Christian VogelbergChristoph Pöhlmann
Nov 19, 2010·European Journal of Oral Sciences·Sabine GroegerJörg Meyle
Feb 11, 2011·Current Infectious Disease Reports·Kevin S W TanPaul A Macary

❮ Previous
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Citations

Oct 2, 2013·Trends in Parasitology·Pauline D Scanlan, Christen R Stensvold
Mar 29, 2014·BMC Infectious Diseases·Dima El SafadiEric Viscogliosi
May 2, 2014·Parasites & Vectors·Helena Lúcia Carneiro SantosHeloisa Werneck de Macedo
Dec 17, 2015·Journal of Huazhong University of Science and Technology. Medical Sciences = Hua Zhong Ke Ji Da Xue Xue Bao. Yi Xue Ying De Wen Ban = Huazhong Keji Daxue Xuebao. Yixue Yingdewen Ban·Xin Fang, Ren-Shi Xu
May 18, 2016·Parasitology International·Sitara S R Ajjampur, Kevin S W Tan
Aug 2, 2016·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Isaac Villegas-GómezOlivia Valenzuela
Aug 29, 2014·Therapeutic Advances in Infectious Disease·Ivan WawrzyniakHicham El Alaoui
Jan 26, 2016·Inflammatory Bowel Diseases·Justin Côté-DaigneaultJean-Frédéric Colombel
Feb 23, 2019·PloS One·Arutchelvan RajamanikamSuresh Kumar
May 11, 2018·The Korean Journal of Parasitology·Maha M A BasyoniDalia Ibrahim Ismail
May 1, 2020·Biomolecules·Patrycja KapczukIrena Baranowska-Bosiacka
Nov 13, 2020·The Pediatric Infectious Disease Journal·Michael W van KalleveenTim G J de Meij
Apr 4, 2021·International Journal of Molecular Sciences·Tracy ParadisFabienne Bon
May 3, 2021·Parasitology International·Claudia Carrera-BravoKevin S W Tan

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