Stationary phase, amino acid limitation and recovery from stationary phase modulate the stability and translation of chloramphenicol acetyltransferase mRNA and total mRNA in Escherichia coli.

Microbiology
Ann E S KuzjJanet L Schottel

Abstract

The functional stability of the chloramphenicol acetyltransferase (cat) mRNA, as well as the functional stability of the total mRNA pool, change during the course of Escherichia coli culture growth. mRNA half-lives are long during lag phase, decrease during the exponential phase and increase again during the stationary phase of the bacterial growth cycle. The half-lives of cat mRNA and total mRNA also increase three- to fourfold during amino acid starvation when compared to exponential culture growth. Even though the stability of the cat message changes about fourfold during culture growth, the amount of cat mRNA per cell mass does not vary significantly between the culture growth phases, indicating that there are compensating changes in cat gene transcription. Translation of cat mRNA also changes during culture growth. In exponential phase, the rate of cat translation is about 14-fold higher than when the culture is in stationary phase. This is in contrast to the fourfold increase in stability of cat mRNA in the stationary-phase culture compared to the exponentially growing culture and indicates that active translation is not correlated with increased mRNA stability. When a stationary-phase culture was diluted into fresh mediu...Continue Reading

References

Jan 2, 1978·European Journal of Biochemistry·M A MinksA R Subramanian
Apr 6, 1978·Molecular & General Genetics : MGG·E SchneiderD Kennell
Nov 9, 1978·Molecular & General Genetics : MGG·S Pedersen, S Reeh
Aug 1, 1976·European Journal of Biochemistry·H R Pelham, R J Jackson
Feb 25, 1975·Journal of Molecular Biology·R S Gupta, D Schlessinger
Aug 15, 1975·European Journal of Biochemistry·R A Laskey, A D Mills
Oct 1, 1992·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·C P EhretsmannH M Krisch
Aug 25, 1990·Nucleic Acids Research·L Krinke, D L Wulff
Jun 1, 1991·Journal of Bacteriology·B J Meyer, J L Schottel
Jan 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·W P Donovan, S R Kushner
Dec 10, 1988·Gene·J G Belasco, C F Higgins
Sep 1, 1974·Journal of Bacteriology·F C NeidhardtD F Smith
Aug 11, 1971·Nature: New Biology·D E Morse, M Guertin
Jul 1, 1983·Analytical Biochemistry·A P Feinberg, B Vogelstein
Apr 1, 1980·Journal of Molecular Biology·M J Casadaban, S N Cohen
Mar 14, 1995·Proceedings of the National Academy of Sciences of the United States of America·E B O'HaraS R Kushner
Jan 1, 1994·Genetica·P AlifanoM S Carlomagno
Mar 24, 1995·Cell·S N Cohen
Aug 1, 1993·Journal of Bacteriology·M P Deutscher
Apr 1, 1993·Journal of Bacteriology·S E ChuangF R Blattner
Jan 1, 1996·Biochimie·L C RaynalA J Carpousis
Mar 1, 1997·Molecular Microbiology·S N Cohen, K J McDowall
Jul 1, 1950·Journal of Bacteriology·B D DAVIS, E S MINGIOLI

❮ Previous
Next ❯

Citations

Mar 30, 2012·Molecular BioSystems·Raúl GuantesVéronique Arluison
Jan 26, 2005·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Li-Yang HsuBan-Hock Tan
Sep 6, 2007·Genome Research·Amy K SchmidNitin S Baliga
Jul 9, 2008·Journal of Bacteriology·Rahul RaghavanMichael F Minnick
Mar 11, 2000·European Journal of Biochemistry·V C WasingerI Humphery-Smith
Nov 17, 2009·Comptes rendus biologies·Flora PicardMuriel Cocaign-Bousquet
Apr 24, 2016·Research in Microbiology·Anisha ViswanathanMeenakshisundaram Kandhavelu
May 5, 2001·Annual Review of Plant Physiology and Plant Molecular Biology·Kirsten Nielsen, Rebecca S Boston
Jun 17, 1999·FEMS Microbiology Reviews·R Rauhut, G Klug

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.