Stationary phase based on cellulose dodecanoate physically immobilized on silica particles for high-performance liquid chromatography

Journal of Chromatography. a
Andressa T VieiraAnizio M Faria

Abstract

The chemical agent free preparation of a stationary phase using a natural macromolecule was the focus of this paper. Thermal immobilization of cellulose dodecanoate on silica particles was used for the preparation of a stationary phase without the use of chemical reagents. Cellulose modification was performed to produce a hydrophobic macromolecule with solubility in common organic solvents. The new stationary phase was characterized morphologically and physico-chemically, presenting as spherical particles immobilized with a thin cellulose dodecanoate layer. The degree of substitution of cellulose dodecanoate was 1.7, which resulted in a separation mechanism in reversed phase mode, but with lower hydrophobicity and higher steric selectivity, which are properties from cellulose. These characteristics resulted in a stationary phase with intrinsic selectivity that was able to separate mixtures of polar drugs, homologs of an anionic surfactant and omeprazole isomers, which are not well resolved in typical C18 phases. Considering that cellulose is a natural polymer and the preparation method of stationary phase involves only physical processes of silica modification, the final material presents as a stationary phase with specific ret...Continue Reading

References

Jan 5, 2002·Journal of Pharmaceutical and Biomedical Analysis·E Francotte, D Huynh
Apr 30, 2005·Angewandte Chemie·Dieter KlemmAndreas Bohn
Nov 10, 2005·Journal of Separation Science·Joseph J Pesek, Maria T Matyska
Sep 15, 2006·Journal of Separation Science·I Wayan MuderawanSiu-Choon Ng
Jan 21, 2010·Journal of Separation Science·Liane Maldaner, Isabel C S F Jardim
Oct 1, 2015·Journal of Separation Science·Szymon BocianBogusław Buszewski
May 4, 1998·Angewandte Chemie·Yoshio Okamoto, Eiji Yashima

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Citations

Mar 3, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Joana TeixeiraCarla Fernandes
Oct 16, 2019·Journal of Separation Science·Yao SunFuhou Lei

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