Stationary phase expression of the arginine biosynthetic operon argCBH in Escherichia coli

BMC Microbiology
Jeevaka P WeerasingheHerb E Schellhorn

Abstract

Arginine biosynthesis in Escherichia coli is elevated in response to nutrient limitation, stress or arginine restriction. Though control of the pathway in response to arginine limitation is largely modulated by the ArgR repressor, other factors may be involved in increased stationary phase and stress expression. In this study, we report that expression of the argCBH operon is induced in stationary phase cultures and is reduced in strains possessing a mutation in rpoS, which encodes an alternative sigma factor. Using strains carrying defined argR, and rpoS mutations, we evaluated the relative contributions of these two regulators to the expression of argH using operon-lacZ fusions. While ArgR was the main factor responsible for modulating expression of argCBH, RpoS was also required for full expression of this biosynthetic operon at low arginine concentrations (below 60 microM L-arginine), a level at which growth of an arginine auxotroph was limited by arginine. When the argCBH operon was fully de-repressed (arginine limited), levels of expression were only one third of those observed in deltaargR mutants, indicating that the argCBH operon is partially repressed by ArgR even in the absence of arginine. In addition, argCBH expres...Continue Reading

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Citations

Aug 21, 2007·Applied and Environmental Microbiology·Qingwei LuoLee R Krumholz
Jun 25, 2019·Journal of Cellular Biochemistry·Muhammad S NadeemAbdul R Shakoori
Dec 29, 2019·BMC Genomics·Tungadri BoseSharmila S Mande
May 4, 2017·The Journal of Maternal-fetal & Neonatal Medicine : the Official Journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians·Abdul Qader Tahir Ismail
Oct 26, 2021·Frontiers in Microbiology·Nadeem JoudehDirk Linke

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Methods Mentioned

BETA
PCR
electrophoresis

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