Statistical analysis of data pertaining to complex state systems by stepwise regression with reformulated parameters; application to spectroscopically monitored hemoglobin oxygen binding data

Biophysical Chemistry
W G Gutheil

Abstract

A method is described for the statistical analysis of data pertaining to complex state systems, based on the concept of reformulating the parameters describing the system as a hierarchy of interactions, and this method demonstrated on the analysis of spectroscopically monitored hemoglobin oxygen binding data [K. Imai, Biophys. Chem. 37 (1990) 197-210]. The concept of reformulation was first extended to state parameters other than delta G degree s, such as the extinction coefficients (epsilon s) associated with different ligation states during hemoglobin oxygen binding. The reformulated parameters are incrementally allowed to vary in the data fitting procedure, and the statistical significance of the added parameters tested by F and Kolmogorov-Smirnov tests. The result of this method is the minimal set of statistically significant parameters required to describe the data. The hierarchical nature of reformulated parameters allows the physical significance of the subset of statistically significant parameters to be discussed even when all reformulated terms may not be statistically significant. Applying this method to hemoglobin oxygen binding data with the reformulated Adair model demonstrated that at least two, and at most three...Continue Reading

References

Jan 1, 1975·Annual Review of Biochemistry·S J Edelstein
Aug 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·G Viggiano, C Ho
Jan 1, 1975·Advances in Protein Chemistry·G Weber
Jan 3, 1992·Science·G K AckersM A Daugherty
Aug 31, 1990·Biophysical Chemistry·D MyersT Yonetani
May 1, 1989·Quarterly Reviews of Biophysics·M F Perutz
Jun 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·S J Edelstein, J T Edsall
Jun 30, 1987·Biochemistry·S J GillC H Robert
Feb 29, 1972·Biochemistry·G Weber
Nov 1, 1967·Proceedings of the National Academy of Sciences of the United States of America·J E Haber, D E Koshland
Nov 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·G Weber
May 1, 1965·Journal of Molecular Biology·J MONODJ P CHANGEUX
Apr 1, 1935·Proceedings of the National Academy of Sciences of the United States of America·L Pauling

❮ Previous
Next ❯

Citations

Jun 5, 2014·Nature·UNKNOWN EXO-200 Collaboration
May 24, 2011·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·Edoardo Milotti
Dec 12, 2012·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·M BotcharovaL Berthouze
Jan 25, 2017·Philosophical Transactions. Series A, Mathematical, Physical, and Engineering Sciences·Momčilo Gavrilov, John Bechhoefer
Jan 11, 2020·Environmental Technology·P T CoutoG A Valdiviesso
Apr 18, 2008·Cognitive Neuropsychology·Andrew C OlsonLiz Halloran
Jul 15, 2015·Journal of Geophysical Research. Space Physics·D SchmidW Baumjohann
Aug 2, 2016·Geophysical Research Letters·D SchmidE L Kepko
Mar 16, 2018·Physical Review Letters·J B AlbertUNKNOWN EXO-200 Collaboration

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.