Statistical analysis of dose-response data from in vitro assays: An illustration using Salmonella mutagenicity data

Toxicology in Vitro : an International Journal Published in Association with BIBRA
R D Combes

Abstract

One of the advantages of in vitro methods is ease of testing multiple concentrations of chemicals for dose responses. A well defined response is used to confirm toxicity, especially for marginal effects, and a significant dose response alone indicates some effect of treatment for further clarification. For sublethal processes (e.g. enzyme induction, organelle damage or mutagenesis), decreases in response can arise at high dose levels that kill cells (cytolethality). This compromises statistical analysis of dose responses using standard approaches, such as ranking, which do not allow for omission of cytolethal dose data. An alternative is the recursive, non-parametric S (M) ( built1 2 )/ Jonckheere-Terpstra test , where omission of results is permitted. Use of this approach is illustrated in conjunction with recommended statistical analyses (Dunnett's 't'-test and Wahrendorf ranking) for non-parametric data from Salmonella mutagenicity assays where the problem is frequently encountered. It is shown that the recursive test can be used for analysing non-parametric dose responses from in vitro assays, where decreases in response are seen at high test chemical concentrations.

Citations

Jun 7, 2014·Journal of Biopharmaceutical Statistics·Joseph WuMark Chang
Dec 15, 2004·Alternatives to Laboratory Animals : ATLA·Frank Bretz, Ludwig A Hothorn
Jan 7, 2003·Alternatives to Laboratory Animals : ATLA·Ludwig A Hothorn
Dec 15, 2004·Alternatives to Laboratory Animals : ATLA·Ludwig A Hothorn, Frank Bretz

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.