Apr 18, 2020

Expanding the diversity of bacterioplankton isolates and modeling isolation efficacy with large scale dilution-to-extinction cultivation

BioRxiv : the Preprint Server for Biology
M. W. HensonCameron Thrash


Cultivated representatives of bacterioplankton from diverse lineages and locations are essential for experimentally determining the physiology and metabolism that influences their distributions and ecological functions. Despite widespread efforts to bring more microorganisms into culture, the large majority of taxa either remain uncultivated or lack isolates from diverse geographic locales. Furthermore, we have little understanding of the factors that influence relative cultivation success for any particular taxon. We paired large scale dilution-to-extinction (DTE) cultivation with microbial community analysis and DTE modeling to expand the phylogenetic and geographic diversity of cultivated bacterioplankton and to evaluate the success of DTE cultivation for isolating abundant taxa. Here we report the results from 17 DTE experiments totaling 7,820 individual incubations over three years that resulted in 328 repeatably transferable cultures. Comparison of isolates to microbial community taxonomic profiles of source waters indicated that we successfully isolated 5% of the observed bacterioplankton taxa across all 17 sites. 43% and 26% of our isolates matched operational taxonomic units (OTUs) and amplicon single nucleotide varian...Continue Reading

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Zika Virus Disease (Disorder)
Zika virus (organism)
Statistical Analysis

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