Statistical Approach for Optimization of Physiochemical Requirements on Alkaline Protease Production from Bacillus licheniformis NCIM 2042.

Enzyme Research
Biswanath Bhunia, Apurba Dey

Abstract

The optimization of physiochemical parameters for alkaline protease production using Bacillus licheniformis NCIM 2042 were carried out by Plackett-Burman design and response surface methodology (RSM). The model was validated experimentally and the maximum protease production was found 315.28 U using optimum culture conditions. The protease was purified using ammonium sulphate (60%) precipitation technique. The HPLC analysis of dialyzed sample showed that the retention time is 1.84 min with 73.5% purity. This enzyme retained more than 92% of its initial activity after preincubation for 30 min at 37°C in the presence of 25% v/v DMSO, methanol, ethanol, ACN, 2-propanol, benzene, toluene, and hexane. In addition, partially purified enzyme showed remarkable stability for 60 min at room temperature, in the presence of anionic detergent (Tween-80 and Triton X-100), surfactant (SDS), bleaching agent (sodium perborate and hydrogen peroxide), and anti-redeposition agents (Na(2)CMC, Na(2)CO(3)). Purified enzyme containing 10% w/v PEG 4000 showed better thermal, surfactant, and local detergent stability.

References

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Citations

Nov 4, 2015·International Journal of Biological Macromolecules·Uma Shankar Prasad UdayBiswanath Bhunia
May 21, 2014·Preparative Biochemistry & Biotechnology·Yun-Zhu XiaoXiang-Yang Gao
Jun 14, 2016·International Journal of Biological Macromolecules·Ahsan SalihiMansour Aliabadian
Sep 6, 2020·Bioprocess and Biosystems Engineering·Nelda L OliveraMarina Nievas
Nov 16, 2021·Environmental Science and Pollution Research International·Hilmi Amanah Aditya CahyaningtyasMamoru Wakayama

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