Statistical exploration of variation in quantitative two-dimensional gel electrophoresis data

Proteomics
John S GustafssonMats Rudemo

Abstract

Two-dimensional gel electrophoresis is a major technique in global analysis at the protein level. This paper presents an examination of spot volume data from three gel sets with radioactively labeled yeast Saccharomyces cerevisiae proteins. A strong variance versus mean dependence in data was found to be stabilized by applying a shifted logarithmic transformation. However, transformed data showed a remaining substantial variance heterogeneity for different proteins. Furthermore, examination of studentized residuals revealed that transformed data were approximately normally distributed and that there were spatial correlations among the measurement errors in the gel.

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Citations

Aug 9, 2012·Omics : a Journal of Integrative Biology·Keith RichardsonJohannes P C Vissers
May 17, 2005·Expert Review of Proteomics·Andrew N StephensElizabeth J Harry
Feb 1, 2006·Expert Review of Proteomics·Asa M Wheelock, Susumu Goto
Jun 16, 2011·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Amy N PerezWendi M David
Jul 22, 2009·Journal of Proteomics·Besma Sghaier-HammamiJesús V Jorrín-Novo
Aug 19, 2007·Proteomics·Tiphaine Monsinjon, Thomas Knigge
Nov 4, 2010·Proteomics·Andrew W DowseyMichael J Dunn
Nov 4, 2006·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Jean-François ChichSylvie Huet
Jan 6, 2007·Journal of Proteome Research·Bruno MeunierJean-François Hocquette
Oct 24, 2007·Journal of Proteome Research·Cristina-Maria Valcu, Mihai Valcu
Apr 16, 2009·Journal of Proteome Research·Harald GroveEllen M Faergestad
Feb 2, 2008·Journal of Proteome Research·Kristina Nedenskov JensenBo M Jørgensen
Oct 11, 2005·Journal of Proteome Research·Craig RowellCoral A Lamartiniere
Jun 15, 2005·Journal of Proteome Research·Sybille M N HuntMarc R Wilkins

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