Statistical Optimization of Culture Variables for Enhancing Agarase Production by Dendryphiella arenaria Utilizing Palisada perforata (Rhodophyta) and Enzymatic Saccharification of the Macroalgal Biomass

Marine Biotechnology
Mohamed GomaaKhayria M Abdel-Gawad

Abstract

Agarase is a promising biocatalyst for several industrial applications. Agarase production was evaluated by the marine fungus Dendryphiella arenaria utilizing Palisada perforata as a basal substrate in semi-solid state fermentation. Seaweed biomass, glucose, and sucrose were the most significant parameters affecting agarase production, and their levels were further optimized using Box-Behnken design. The maximum agarase activity was 7.69 U/mL. Agarase showed a degree of thermostability with half-life of 99 min at 40 °C, and declining to 44.72 min at 80 °C. Thermodynamics suggested an important process of protein aggregation during thermal inactivation. Additionally, the enzymatic saccharification of the seaweed biomass using crude agarase was optimized with respect to biomass particle size, solid/liquid ratio, and enzyme loadings. The amount of biosugars obtained after optimization was 26.15 ± 1.43 mg/g. To the best of our knowledge, this is the first report on optimization of agarase in D. arenaria.

References

Jan 1, 1997·Bioscience, Biotechnology, and Biochemistry·R KobayashiS Usami
Dec 1, 2005·Applied Microbiology and Biotechnology·Jingxue WangHuashi Guan
Jun 2, 2009·Marine Biotechnology·Mengxian LongXun Xu
Oct 1, 2010·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Chulhong OhJehee Lee
Aug 16, 2015·Bioresource Technology·Eun Ju YunKyoung Heon Kim

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BETA
enzymatic saccharification

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BBD
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