Statistical optimization of medium components for production of extracellular chitinase by Basidiobolus ranarum: a novel biocontrol agent against plant pathogenic fungi

Journal of Basic Microbiology
P MishraS K Singh

Abstract

The influence of concentration of medium components such as colloidal chitin, lactose, malt extract, yeast extract, and peptone on the chitinase production from Basidiobolous ranarum at the flask level were studied by using statistical tool Central Composite Design (CCD) and analysed by Response Surface Methodology (RSM). The results revealed that colloidal chitin, malt extract and peptone had significant effect (P < 0.01) on the chitinase production at their individual levels. The polynomial equation of the model developed incorporates 3 linear, 3 quadratic and 5 interactive terms. Maximum chitinase production of 3.47 U ml(-1) was achieved with 1.5% colloidal chitin, 0.125% lactose, 0.025% malt extract and 0.075% peptone. After optimization, chitinase activity was increased by 7.71 fold. A second order polynomial equation was found to be useful for the development of efficient bioprocess for chitinase production. To screen the biotechnological potential of this enzyme, degradation of fungal mycelia by ammonium sulphate precipitate of the same was studied for several pathogenic fungi-in vitro which showed promising results particularly against Rhizoctonia solani and Fusarium solani. This study provides the first evidence showin...Continue Reading

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Citations

Aug 31, 2013·Current Microbiology·Maria Swiontek BrzezinskaMaciej Walczak
Nov 25, 2014·Natural Products and Bioprospecting·Aarti Thakkar, Meenu Saraf
May 1, 2019·Molecular Plant-microbe Interactions : MPMI·Zhiqin LiuShuilin He
May 11, 2017·International Journal of Biological Macromolecules·Manish KumarNidhi Pareek

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