Statistical optimization of process variables for the production of an anticancer drug (colchicine derivatives) through fermentation: at scale-up level

New Biotechnology
Kashyap Kumar Dubey, B K Behera

Abstract

Effect of various nutritional components and fermentation parameters on biotransformation of colchicine using Bacillus megaterium ACBT03 to produce 3-demethylated colchicine (3-DMC) has been investigated. Initially, Plackett-Burman (PB) design was used to evaluate the 11 nutritional variables to screen medium component that significantly influences the 3-demethylated colchicine (3-DMC) production. Three most statistically significant nutrients that is starch, glycerol and yeast extract were identified. Response surface methodology was used to optimize fermenter operational factors pH, temperature, impeller tip velocity and aeration rate for high 3-DMC production. The predicted results of 3-DMC yield (7689.13mg/l) were obtained at 5-l-jar fermenter after optimization of pH 6.8, temperature 28°C, impeller tip velocity 4650cm/min, and aeration rate 2.0vvm. The validity of model was confirmed; and the optimum medium designed led a maximum 3-DMC yield of 7335mg/l, which had 3-4% variation with predicted results. Furthermore, the experiment was scaled up in the 70-l fermenter.

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Citations

Aug 16, 2011·New Biotechnology·Aydin BerenjianFariba Dehghani
Apr 30, 2017·Applied Biochemistry and Biotechnology·Chao ZhangJian Zhang
Feb 11, 2021·Artificial Cells, Nanomedicine, and Biotechnology·Manlika PhayaPitchaya Mungkornasawakul

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